Nucleic acid isolation from stool samples and other...

Details Nucleic acid isolation from stool samples and other... Nucleic acid isolation from stool samples and other...

2006-02-28

2006-02-28

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

07005266

ABSTRACT:
The invention relates to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out said method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infections, in particular, bacterial or viral infections, or mutations, in particular, for tumour-specific DNA mutations.

REFERENCES:
patent: 4935342 (1990-06-01), Seligson et al.
patent: 6084091 (2000-07-01), Muller et al.
patent: DE 199 06 385 (2000-08-01), None
patent: WO 93/20235 (1993-10-01), None
patent: WO 95/01359 (1995-01-01), None
patent: WO 97/07239 (1997-02-01), None
Wan et al. A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton Analytical Biochemistry vol. 223 pp. 7-12 1994.
Chung et al.,Chem. Abstr., 127: 146699x (1997).
de Kok et al.,Clin. Chem.,, 44: 2201-2204 (1998).
Deuter et al.,Nucleic Acids Res., 23: 3800-3801 (1995).
Hopwood et al.,Int. J. Legal Med., 108: 237-243 (1996).
Monteiro et al.,J. Clin. Microbiol., 35: 995-998 (1997).
Pantosti et al.,J. Clin. Microbiol., 35: 2482-2486 (1997).
Ramamurthy et al.,J. Clin. Microbiol., 31: 3068-3070 (1993).
Sidransky et al.,Science, 256: 103-105 (1992).
Sivolap et al.,Chem. Abstr., 117: 20600lu (1992).
Tuchili et al.,J. Vet. Med. Sci., 58: 881-884 (1996).
Uwatoko et al.,Vet. Microbiol., 52: 73-79 (1996).
van Zwet et al.,J. Clin. Microbiol., 32: 1346-1348, (1994).
Wang and Brown,Anal. Biochem. 269: 198-201 (1999).
Wilde et al.,J. Clin. Microbiol., 28: 1300-1307, (1990).

Affiliated with

Also associated with

Rating

Nucleic acid isolation from stool samples and other... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid isolation from stool samples and other..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid isolation from stool samples and other... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3669608