Nucleic acid isolation and purification

Details Nucleic acid isolation and purification Nucleic acid isolation and purification

1997-10-31

2000-08-29

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2541, 422 99, 203 1, 203 2, 203 34, 203 35, 204193, 204194, 20429825, 204400, 204403, C07H 2100

Patent

active

061110965

ABSTRACT:
The invention is based on the discovery that hyperbaric, hydrostatic pressure reversibly alters the partitioning of nucleic acids between certain adsorbed and solvated phases relative to partitioning at ambient pressure. The new methods and devices disclosed herein make use of this discovery for highly selective and efficient, low salt isolation and purification of nucleic acids from a broad range of sample types, including forensic samples, blood and other body fluids, and cultured cells.

REFERENCES:
patent: 3609252 (1971-09-01), Broce et al.
patent: 5003047 (1991-03-01), Yarmush et al.
patent: 5934766 (1999-08-01), Feigel et al.

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