Nucleic acid isolation

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S025410, C536S025420, C435S320100, C435S259000, C435S006120, C435S091100

Reexamination Certificate

active

07129344

ABSTRACT:
A method for isolating plasmids DNA from a DNA containing material which comprises plasmid DNA and genomic DNA, comprising extracting the plasmid DNA into a water-immiscible organic solvent, a chaotrope and water under conditions to denature the genomic DNA and recovering the plasmid DNA from the organic phase.

REFERENCES:
patent: 5075430 (1991-12-01), Little
patent: 5637687 (1997-06-01), Wiggins
patent: 5643767 (1997-07-01), Fischetti et al.
patent: 6383393 (2002-05-01), Colpan et al.
patent: 0 281 390 (1988-09-01), None
patent: 0 366 438 (1990-05-01), None
patent: 0 376 080 (1990-07-01), None
patent: 0 517 515 (1992-12-01), None
patent: 03101688 (1991-04-01), None
patent: WO 87/07645 (1987-12-01), None
patent: WO 91/02089 (1991-02-01), None
patent: WO 95/01359 (1995-01-01), None
patent: WO 95/02049 (1995-01-01), None
patent: WO 99/61603 (1999-12-01), None
Sawadogo and Dyke, NAR, 1991, p. 19(3), p. 674.
SCHP-445, Mutual Solubility of Liquids in a Binary Two-Phase System, Chemical Thermodynamics Lab, downloaded May 3, 2005.
Nelson et al., Purification of Cloned and Genomic DNA by Guanidine Thiocyanate/Isobutyl Alcohol Fractionation, Analytical Biochemistry 207, 197-201 (1992).
Sambrook et al., Molecular Cloning, a Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, 1.22-1.24, 9.48, & E.12-E-14 (1989).
Voet et al., Biochemistry, Chapter 28, pp. 805-807, 815-816, & 844.
Old et al., Studies in Microbiology, vol. 2, Principles of Gene Manipulation, An Introduction to Genetic Engineering, Chapter 1 Section 1, pp. 7-10 & Chapter 3, Section 2, pp. 43-44.
Watson et al., Molecular Biology of the Gene, Fourth Edition, pp. 243-244.
Chomczynski, “A Reagent for the Single-Step Simulataneous Isolation of RNA, DNA and Proteins from Cell and Tissue Samples”, BioTechniques, 15:3:532-536 (1993).
Chomszynski and Sacchi, “Single-Step Method of RNA Isolation by Acid Guanidinium Thlocyanate-Phenol-Chloroform Extraction” Analytical Biochemistry, 162:156-159 (1987).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Nucleic acid isolation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid isolation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid isolation will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3677115

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.