Nucleic acid hybridization assay employing probes crosslinkable

Details Nucleic acid hybridization assay employing probes crosslinkable Nucleic acid hybridization assay employing probes crosslinkable

1983-12-12

1986-07-08

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, 436501, C12Q 168, G01N 33566

Patent

active

045993033

ABSTRACT:
Novel methods and reagents for determining the presence of specific nucleic acid base sequences by employing crosslinking reactions of unique molecules capable of forming covalent bonds which are bonded with various labels or ligands for amplication.
Single stranded nucleic acid probes are employed which contain complementary base sequences to nucleic acid target molecules. By first hybridizing and then forming covalent bonds between the probe and target the amount of label in the crosslinked hybrid can be measured as an extremely sensitive method for assaying for specific nucleic acid sequences.

REFERENCES:
patent: 3798131 (1974-03-01), Rounds et al.
Ashley et al, Analytical Biochem., 140(1984) 95-103.
Dutton et al., Analytical Biochem., 140(1984) 121-128.
Griffith et al, J. Mol. Biol., 157(1982) 321-330.
Wu et al., Proc. Natl. Acad. Sci. USA, 78(1981) 7059-63.
Wollenzien, P. L. and Cantor, C. R., "Marking the Polarity of RNA Molecules for Electron Microscopy by Covalent Attachment of Psoralen-DNA Restriction Fragments", Proc. Natl. Acad. Sci. USA, 79:3940-3944 (1982).

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