Nucleic acid fragments encoding stereospecific nitrile hydratase

Details Nucleic acid fragments encoding stereospecific nitrile hydratase Nucleic acid fragments encoding stereospecific nitrile hydratase

1996-10-04

1998-09-22

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435228, 435232, 43525233, 4352542, 43525423, 4353201, 435877, 536 232, 536 234, C12N 120, C12N 980, C12N 988, C07H 2104

Patent

active

058112863

ABSTRACT:
The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.

REFERENCES:
patent: 4366250 (1982-12-01), Jallageas et al.
patent: 5034329 (1991-07-01), Cerbelaud et al.
patent: 5238828 (1993-08-01), Murakami
patent: 5314819 (1994-05-01), Yamada et al.
Azza, S. et al, "Cloning of the wide spectrum amidase gene from Brevibacterium sp. R312 by genetic complementation. Overexpression in Brevibacterium sp. and Escherichia coli", FEMS Microbiol. Lett., 122, 129-136 (1994).
Kobayashi, M. et al, "Amidase coupled with low-molelcular-mass nitrile hydratase from Rhodococcus rhodochrous J1", Eur. J. Biochem., 217, 327-336 (1993).
Blakey, A.J. et al, "Regio- and sterio-specific nitrile hydrolysis by the nitrile hydratase from Rhodococcus AJ270", FEMS Microbiology Lett., 129, 57-62 (1995).
Ikehata, O. et al, "Primary strucure of nitrile hydratase deduced from the nucleotide sequence of a Rhodococcus species and its expression in Escherichia coli", Eur. J. Biochem., 181, 563-570 (1989).
Nishiyama, M. et al, "Cloning and Characterization of Genes Responsible for Metabolism of Nitrile Compounds from Pseudomonas chloroaphis B23", J. of Bacteriology, 173(8), 2465-2472 (1991).
Kobayashi, M. et al, "Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt-containing nitrile hydratase genes", Biochimica et Biophysica Acta., 1129, 23-33 (1991).
Mayaux, J. et al, "Purification, Cloning, and Primary Structure of an Enantiomer-Selective Amidase from Brevibacterium sp. Strain R312: Structural Evidence for Genetic Coupling with Nitrile Hydratase"., J. Bacteriol., 172(12), 6764-6773 (1990).
Hashimoto et al., "Nitrile hydratase gene from Rhodococcus sp. N-774 requirement for its downstream region for efficient expression", Bioscience, Biotechnology and Biochemistry, vol. 58, pp. 1859-1865, Oct., 1994.

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