Nucleic acid extraction methods

Details Nucleic acid extraction methods Nucleic acid extraction methods

1987-09-04

1990-03-13

Rosen, Sam

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435269, 536 27, C12N 108

Patent

active

049083180

ABSTRACT:
A method of extracting nucleic acids from a crude buffy coat fraction in an aqueous medium includes the steps of treating the buffy coat fraction to break open the white blood cells to release the proteins and the nucleic acids in the cells into the aqueous medium; solubilizing the proteins and the nucleic acids in the aqueous medium; and precipitating the nucleic acids under conditions under which the proteins remain in solution.

REFERENCES:
patent: 3433782 (1969-03-01), Kreiser
patent: 3582468 (1971-06-01), Birnbaum
Kochetkov et al.--Organic Chemistry of Nucleic Acids, (Part A), (1971), Plenum Press, pp. 17 and 18.

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