Nucleic acid enzyme for RNA cleavage

Details Nucleic acid enzyme for RNA cleavage Nucleic acid enzyme for RNA cleavage

2006-12-12

2006-12-12

Zara, Jane (Department: 1635)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091100, C536S023100, C536S024300, C536S024310, C536S024320, C536S024330, C536S024500

Reexamination Certificate

active

07148044

ABSTRACT:
A method is described for cleaving a nucleic acid substrate with a nucleic acid enzyme at a cleavage site comprising mixing the substrate with the enzyme, wherein the substrate includes a 7 nucleotide sequence with at least 6 nucleotides 3′ to the cleavage site and at least 1 nucleotide 5′ to the cleavage site and of formula:in-line-formulae description="In-line Formulae" end="lead"?5′-H′GNNHNN-3′in-line-formulae description="In-line Formulae" end="tail"?wherein each N is a nucleotide which may be the same or different, H is a nucleotide selected from the group consisting of A, U, C, and T, and is the site of cleavage, and H′ is a ribonucleotide selected from the group consisting of A, U, and C, wherein (i) the first nucleotide 3′ to the cleavage site is capable of forming a wobble pair with the enzyme, (ii) the second, third, fifth, and sixth nucleotides 3′ to the cleavage site are capable of forming conventional Watson-Crick base pairs with the enzyme, (iii) the fourth nucleotide 3′ to the cleavage site is capable of forming a non-conventional Watson-Crick base pair with the enzyme, and (iv) the first nucleotide 5′ to the cleavage site does not form a base pair with the enzyme; and the enzyme comprises a substrate binding portion which is capable of base pairing to the 6 nucleotides 3′ to the cleavage site of the substrate and which binding portion comprises the sequence:in-line-formulae description="In-line Formulae" end="lead"?3′-UNNXNN-5′in-line-formulae description="In-line Formulae" end="tail"?wherein each N is a nucleotide which may be the same or different, and X is a nucleotide selected from the group consisting of T, U, A, and G, whereby binding of the substrate to the enzyme effects cleavage of the substrate at the cleavage site.

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