Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2007-07-23
2010-12-14
Burkhart, Michael (Department: 1633)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S320100, C435S235100, C435S325000, C435S070100, C435S455000
Reexamination Certificate
active
07851607
ABSTRACT:
The present invention relates to a new polynucleotide that encodes a polypeptide involved in cellular entrance of PRRSV, to a recombinant vector comprising said polynucleotide, to a cell capable of expressing said polypeptide, a method of producing said polypeptide as well as to cell culture and to a novel method of producing the PRRSV virus. The present invention further relates to a method of identifying compounds that affect the PRRSV receptor function of the polypeptide as well as to the use of the polypeptide or identified compounds in the manufacture of medicaments. The present inventors have succeeded in isolating a protein from PAM membranes that seems to play a crucial role in virus entry into the cell. The elucidated nucleotide sequence encoding the protein, as well as the amino acid sequence of the protein, were compared with sequences stored in sequence databases. Surprisingly the putative PRRSV receptor provided by the present invention showed a great deal of homology to certain proteins belonging to the Siglec family.
REFERENCES:
patent: 2004/0248124 (2004-12-01), Pensaert et al.
patent: 2009/0104147 (2009-04-01), Delputte et al.
patent: WO03010200 (2003-02-01), None
Vanderheijden, N. et al., “Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar Macrophages,” J. Virology, vol. 77, No. 15, p. 8207-8215 (2003).
Nauwynck, H. et al., “PRRSV-macrophage interaction and putative receptors,” Veterinary Research, vol. 31, No. 1, p. 22 (Jan. 2000).
Therrien, D. et al., “Preliminary characterization of protein binding factor for porcine reproductive and respiratory syndrome virus on the surface of permissive and non-permissive cells,” Archives of Virology, vol. 145, p. 1099-1116 (2000).
Nauwynck, H. et al., “Entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages via receptor-mediated endocytosis,” Journal of General Virology, vol. 80, p. 297-305 (1999).
Duan, X. et al., “Identification of a Putative Receptor for Porcine Reproductive and Respiratory Syndrome Virus on Porcine Alveolar Macrophages,” Joumal of Virology, vol. 72, No. 5, p. 4520-4523 (May 1998).
Duan, X., et al., “Porcine reproductive and respiratory syndrome virus Infection of alveolar macrophages can be blocked by monoclonal antibodies against cell surface antigens,” Advances in Experimental Medicine and Biology, vol. 440, p. 81-88 (1998).
Duan, X., et al., “Effects of origin and state of differentiation and activation of monocytes/macrophages on their susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV),” Archives of Virology, vol. 142, p. 2483-2497 (1997).
Crocker, P., et al., “Sialoadhesin, a macrophage sialic acid binding receptor for haemopoietic cells with 17 immunoglobulin-like domains,” The EMBO Joumal, vol. 13, No. 19, p. 4490-4503 (1994).
Ngo, J. et al., “Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox,” from “The Protein Folding Problem and Tertiary Structure Prediction,” Birkhauser Boston, p. 491-495 (1994).
Rudinger, J., “Characteristics of the amino acids as components of a peptide sequence,” in “Peptide Hormones,” University Park Press, p. 1-7 (1976).
Frishman et al., Seventy-Five Percent Accuracy in Protein Secondary Structure Prediction, Proteins: Structure, Function and Genetics, 1997, pp. 329-335, vol. 27.
Nauwynck Hans
Pensaert Maurice
Vanderheijden Nathalie
Burkhart Michael
TraskBritt
Universiteit Gent
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