Nucleic acid encoding novel protein domain which binds tyrosine

Details Nucleic acid encoding novel protein domain which binds tyrosine Nucleic acid encoding novel protein domain which binds tyrosine

1995-11-01

1999-07-20

Spector, Lorraine

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 235, 536 234, 536 241, 536 2431, 435 691, 4353201, 435325, 435348, 4352523, 43525233, 4352541, 530350, C12N 1512, C12N 510

Patent

active

059255471

ABSTRACT:
The present invention generally relates to novel protein domains which bind to tyrosine-phosphorylated proteins. More particularly, the present invention provides non-SH2 protein domains that bind phosphotyrosine. The present invention also provides various composite polypeptides comprising these domains, such that these composite polypeptides retain the ability to bind tyrosine phosphorylated proteins. It also provides nucleic acids encoding these polypeptides. Typically, these nucleic acids may also comprise a promoter for expression, and a segment encoding fusion peptides on the amino or carboxy terminus of the expressed composite protein. Also included within the present invention are methods of preparing these polypeptides and cells capable of expressing them. Also provided are methods of using these polypeptides in research, diagnostic and therapeutic applications.

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