Nucleic acid encoding fluorescent proteins from aquatic species

Details Nucleic acid encoding fluorescent proteins from aquatic species Nucleic acid encoding fluorescent proteins from aquatic species

2002-12-09

2008-08-19

Robinson, Hope A (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S006120, C435S007100, C435S320100, C435S252000

Reexamination Certificate

active

07413874

ABSTRACT:
Provided are four new fluorescent proteins. The proteins were derived from two wild-type fluorescent proteins: a red fluorescent protein (RFP) that was isolated fromActinodiscusorDiscosomasp. 1 and a green fluorescent protein (GFP) isolated fromMontastraea cavernosa. Two mutant forms were generated from each wild-type protein. Each of the mutated forms has a higher fluorescence intensity than the respective wild-type form. The mutant forms of the fluorescent proteins allow for more sensitive detection of the fluorescence emitted by the proteins. Additionally, one of the mutant proteins is more resistant to photobleaching than its wild-type protein. The invention also encompasses isolated nucleic acids encoding the mutant forms of the wild-type RFP and GFP.

REFERENCES:
patent: 4968613 (1990-11-01), Masuda et al.
patent: 5096825 (1992-03-01), Barr et al.
patent: 5182202 (1993-01-01), Kajiyama et al.
patent: 5219737 (1993-06-01), Kajiyama et al.
patent: 5229285 (1993-07-01), Kajiyama et al.
patent: 5283179 (1994-02-01), Wood
patent: 5292658 (1994-03-01), Cormier et al.
patent: 5330906 (1994-07-01), Kajiyama et al.
patent: 5352598 (1994-10-01), Kajiyama et al.
patent: 5418155 (1995-05-01), Cormier et al.
patent: 5583024 (1996-12-01), McElroy et al.
patent: 5604123 (1997-02-01), Kazami et al.
patent: 5641641 (1997-06-01), Wood
patent: 5650289 (1997-07-01), Wood
patent: 5670356 (1997-09-01), Sherf et al.
patent: 5674713 (1997-10-01), McElroy et al.
patent: 5700673 (1997-12-01), McElroy et al.
patent: 5744320 (1998-04-01), Sherf et al.
patent: 5786464 (1998-07-01), Seed et al.
patent: 5795737 (1998-08-01), Seed et al.
patent: 5874304 (1999-02-01), Zolotukhin et al.
patent: 5952547 (1999-09-01), Cornelissen et al.
patent: 5968750 (1999-10-01), Zolotukhin et al.
patent: 5976796 (1999-11-01), Szalay et al.
patent: 6020192 (2000-02-01), Muzyczka et al.
patent: 6074859 (2000-06-01), Hirokawa et al.
patent: 6169232 (2001-01-01), Hey et al.
patent: 6342379 (2002-01-01), Tsien et al.
patent: 6414119 (2002-07-01), Fisher
patent: 6689391 (2004-02-01), Goswami et al.
patent: 6723537 (2004-04-01), Peelle
patent: 6900304 (2005-05-01), Tsien et al.
patent: 2002/0064842 (2002-05-01), Sorge et al.
patent: 2002/0107362 (2002-08-01), Thastrup et al.
patent: 2003/0106078 (2003-06-01), Falkowski et al.
patent: 2003/0157643 (2003-08-01), Almond et al.
patent: 2005/0032085 (2005-02-01), Labas et al.
patent: WO 95/25798 (1995-09-01), None
patent: WO 96/22376 (1996-07-01), None
patent: WO 97/26366 (1997-07-01), None
patent: WO 97/28261 (1997-08-01), None
patent: WO 99/14336 (1999-03-01), None
patent: WO 00/34526 (2000-06-01), None
patent: WO 01/27150 (2001-04-01), None
patent: WO 01/62919 (2001-08-01), None
patent: WO 02/16944 (2002-02-01), None
patent: WO 01/094992 (2002-11-01), None
patent: WO 03/042401 (2003-05-01), None
patent: WO 03/042401 (2003-05-01), None
Liu et al.,Is Green Fluorescent Protein Toxic to the Living Cells?, May 1999, Biochemical and Biophysical Research Communications 260, pp. 712-717.
Chalfie et al.,Green Fluorescent Protein as a Marker for Gene Expression, Feb. 1994, Science, vol. 263, pp. 802-805.
Fautin et al.,Two New Species of Deep-Water Corallimorpharia(Cnidaria:Anthozoa)from the Northeast Pacific, Corallimorphus denhartogi and C.pilatus, Apr. 2002, Pacific Science, vol. 56, No. 2:113-124, pp. 113-124.
Yang et al.,The Molecular Structure of Green Fluorescent Protein, Oct. 1996, Nature Biotechnology, vol. 14, pp. 1246-1251.
Yokoe et al.,Spatial Dynamics of GEP-tagged Proteins Investigated by Local Fluorescence Enhancement, Oct. 1996, Nature Biotechnology, vol. 14, pp. 1252-1256.
Ormo et al.Crystal Structure of the Aequorea Victoria Green Fluorescent Protein, Sep. 1996, Science, vol. 273, pp. 1392-1395.
Miyawaki,Green Fluorescent Protein-like Proteins in Reef Anthozoa Animals, 2002, Cell Structure and Function 27, pp. 343-347.
Gibbs et al.,GFP as a Genetic Marker Scorable Throughout the Life Cycle of Transgenic Zebra Fish, Mar./Apr. 2000, Marine Biotechnology, vol. 2, No. 2, pp. 126.
Wiehler et al.,Mutants of Discosoma Red Fluorescent Protein with a GFP-like Chromophore, FEBS Letters 487 (2001), pp. 384-389.
Fradkov et al.,Novel Fluorescent Protein from Discosomao Coral and its Mutants Possessa a Unique Far-Red Fluorescence, FEBS Letters 479 (2000), pp. 127-130.
Matz et al.,Fluorescent Proteins from Nonbioluminescent Anthozoa Species, Nature Biotechnology, vol. 17, Oct. 1999, pp. 969-973.
Clontech,Living Colors Red Fluorescent Protein, CLONTECHniques, Oct. 1999, pp. 1-5.
Prasher et al.,Primary Structure of the Aequorea victoria Green-Fluorescent Protein, 1992, Gene, pp. 229-233.
Dove et al.,Major Colour Patterns of Reef-building Corals are Due to a Family of GFP-like Proteins, 2001, Coral Reefs, pp. 197-204.
James A. Wells, Additivity of Mutational Effects in Proteins, Biochemistry, Sep. 18, 1990, pp. 8511-8517, vol. 29, No. 37, American Chemical Society, U.S.
Seffernick, et al., Melamine Deaminase and Atrazine Chlorohydroalase: 98 Percent Identical but Functionally Different, Journal of Bacteriology, Apr. 2001, p. 2405-2410.
GenBank Accession No. AF406766. (Sep. 2001).
GenBank Accession No. AY037768. (May 2002).
GenBank Accession No. AY037769. (Apr. 2002).
Alberts, “Intron sequences are removed as lariat-shaped RNA molecules,” Molecular Cell Biology, 3d Ed., p. 373 (1994).
Andrews, “Hierarchy of polyadenylation site usage by bovine papillomavirus in transformed mouse cells,” J. Virology, 67:12, pp. 7705-7710 (Dec. 1993).
Aota, “Codon usage tabulated from the GenBank genetic sequence data,” Nucleic Acids Research, v. 16 Suppl, pp. 315-402 (1988).
Benzakour, “Evaluation of the use of the luciferase-reporter-gene system for gene-regulation studies . . . ” Charter Molecular Genetics Lab, p. 385-387 (1995).
Cameron, “Recent advances in transgenic technology,” Molecular Biotechnology, v. 7, pp. 253-265 (1997).
Chen, “Gene transfer and expression in oligodendrocytes under the control of myelin basic protein transcriptional control region . . . ” Gene Therapy, 5:50-58 (1998).
Coker, “8-Br-cAMP inhibits the transient expression of firefly luciferase,” FEBS Letters, 249:2, pp. 183-185 (1989).
Faisst, “Compilation of vertebrate-encoded transcription factors,” Nucleic Acids Research, 20:1, pp. 3-26 (1992).
Genbank AF384683, Montastraea cavernosa green fluorescent protein mRNA (2001).
Genbank AY037770, Montastraea cavernosa mcavFP—7.5 mRNA (2002).
Genbank AY056460, Montastraea cavernosa cyan fluorescent protein mRNA (2001).
Glass, “Gene functions:E. coliand its heritable elements,” RNA and Protein Production, U. Cal. Press, p. 95 (1982).
Gruber, “Design strategy for synthetic luciferase reporter genes,” abstract, 1.1th Symposium on Bioluminescence & Chemiluminescence (2000).
Henning, “Humanizing the yeast telomerase template,” Proc. Natl. Acad. Sci. USA, v. 95, pp. 5667-5671 (May 1998).
Hsieh, “Nucleotide sequence, transcriptional analysis, and glucose regulation of the phenoxazinone synthase gene (phsA) . . . ,” J. Bacteriol. 177:20, pp. 5740-5747 (1995).
Jensen, “The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters,” Appl.&Envir. Microbio, 64:1, pp. 82-87 (1998).
Kappel, “Regulating gene expression in transgenic animals,” Current Opin. in Biotech. 3:548-553 (1992).
Kozak, An analysis of 5′-noncoding sequences from 699 vetebrate messenger RNAs, Nucleic Acids Research, 15:20, pp. 8125-8132 (1987).
Labas, “Diversity and evolution of the green fluorescent protein family,” PNAS, 99:7, pp. 4256-4261 (2002).
Lisser, Compilation ofE. colimRNA promoter sequences, Nucleic Acids Research 21:7, pp. 1507-1516. (1993).
Liu, “Improved assay sensitivity of an engineered secretedRenilla luciferase,” Gene, 237: 153-159 (1999).
Lodish, “Spliceosomes, assembled from snRNPs and a Pre-mRNA, carry out splicing,

Affiliated with

Also associated with

Rating

Nucleic acid encoding fluorescent proteins from aquatic species does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid encoding fluorescent proteins from aquatic species, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid encoding fluorescent proteins from aquatic species will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4009469