Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1995-06-01
1997-04-29
Wax, Robert A.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
4353201, 435357, 536 234, C12N 516, C12N 1563, C12N 1562, C07H 2104
Patent
active
056248377
ABSTRACT:
Chimeric complement inhibitor proteins are provided which include a first functional domain (first amino acid sequence) having C3 inhibitory activity and a second functional domain (second amino acid sequence) having C5b-9 inhibitory activity. The first functional domain is amino terminal to the second functional domain. In this way, the chimeric protein exhibits both C3 and C5b-9 inhibitory activity. The other orientation, i.e., the orientation in which the second amino acid sequence is amino terminal to the first amino acid sequence, only produces C3 inhibitory activity. Nucleic acid molecules encoding such proteins are also provided.
REFERENCES:
patent: 5242810 (1993-09-01), Maraganore et al.
patent: 5338669 (1994-08-01), Gilles et al.
Adams, et al., 1991, "Contribution of the Repeating Domains of Membrane Cofactor Protein (CD46) of the Complement System to Ligand Binding and Cofactor Activity" Journal of Immunology 147:3005-3011.
Albrecht, et al., 1992. "Herpesvirus Saimiri Has a Gene Specifying a Homologue of the Cellular Membrane Glycoprotein CD59" Virology 190:527-530.
Coyne et al., 1992. "Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor," Journal of Immunology 149:2906-2913.
Davies, et al., 1989. "CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the acton of the complement membrane attack complex on homologous cells" J. Exp. Med. 170:637-654.
Lublin, et al., "Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP)," J. Exp. Med., 168:181-194, 1988.
Lublin, et al., "Decay-accelerating factor: Biochemistry, molecular biology, and function," Ann. Rev. Immunol., 7:35-38, 1989.
Lublin, et al,. "Phospholipid-anchored and Transmembrane Versions of Either Decay-accelearating Factor or Membrane Cofactor Protein Show Equal Efficiency in Protection from Complement-mediated Cell Damage," J. Exp. Med., 174:35-44, 1991.
Meri, et al., 1990- "Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers" Immunology 71:1-9.
Moran, et al., 1992. "Human recombinant soluble decay accelerating factor inhibits complement activation in vitro and in vivo," J. Immunol. 410:1736-1743.
Norris, et al., 1993. "Structure-Function Relationships of CD59" Blood, 82(Suppl.):202a.
Okada, et al., 1989. "Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement" J. Immunol. 143: 2262-2266.
Perkins et al., 1988. "A Study of the Structure of Human Complement Component Factor H by Fourier Transform Infrared Spectroscopy and Secondary Structure Averaging Methods," Biochemistry, 27:4004-4012.
Petranka, et al., 1993, "The Structure and Function of CD59: The Importance of the Disulfide Structure and Identification of a Primary Epitope," Molec. Immunol. 30(suppl. 1):44.
Philbrick, et al., 1990. "The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility" Eur. J. Immunol. 20:87-92.
Rollins, et al., 1990. "The complement-inhibitory activity of CD59 resides in its capacity to block incorporation of C9 into membrane C5b-9" J. Immunol. 144:3478-3483.
Rollins, et al., 1991. "Inhibition of homologous complement by CD59 in mediated by a species-selective recognition conferred through binding to C8 within C5b-8 or C9 within C5b-9" J. Immunol. 146:2345-2351.
Rother, et al., 1994. "Inhibition of Complement-Mediated Cytolysis by the Terminal Complement Inhibitor of Herpesvirus Saimiri," J. Virol. 68:730-737.
Sawada, et al., 1990. "Isolation and expression of the full-length cDNA encoding CD59 antigen of human lymphocytes" DNA and Cell. Biol. 9: 213-220.
Stefanova, et al., 1989. "Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol" Mol. Immunol. 26:153-161.
Su, et al. 1991. "The Glycolsyl Phosphatidylinositol Anchor Is Critical for Ly-6A/E-mediated T Cell Activation," J. Cell Biol. 112:377-384.
Tone, et al., 1992. "Gene structure of human CD59 and demonstration that discrete mRNAs are generated by alternative polyadenylation" J. Mol. Biol. 227:971-976.
Venneker, et al., 1992. "CD59: a molecule involved in antigen presentation as well as downregulation of membrane attack complex" Exp. Clin. Immunogenet. 9:33-47.
Walsh, et al., 1991. "Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement" Eur. J. Immunol. 21:847-850.
Whitlow, et al., 1990. "H19, a surface membrane molecule involved in T-cell activation, inhibits channel formation by human complement" Cell. Immunol. 126:176-184.
Wing, et al., 1992. "Oligodendrocytes lack glycolipid anchored proteins which protect them against complement lysis. Restoration of resistance to lysis by incorporation of CD59" Immunology 76:140-145.
Zhao, et al., 1991. "Amplified gene expression in CD59-transfected Chinese hamster ovary cells confers protection against the membane attack complex of human complement" J. Biol. Chem. 266: 13418-13422.
Curtis et al., US, A, 5,073,627 "Fusion Proteins Comprising GM-CSF and IL-3" 17 Dec. 1991.
Reid et al., Immunology Today, vol. 7, pp. 230-234 "Complement System Proteins Which Interact With C3b or C4b" 1986.
Sims et al., US, A, 5,135,916 "Inhibition of Complement Mediated Inflammatory Response" 4 Aug. 1992.
Wagner et al., US, A, 4,873,191 "Genetic Transformation of Zygotes" 10 Oct. 1989.
Weisman et al., Science vol. 249, pp. 146-151 "Soluble Human Complement Receptor Type 1: in vivo inhibitor of complement suppressing post-ischemic myocardial inflammation and necrosis" 1990.
White et al,. WO, A, 91/05855 "Modified Biological Material" 2 May 1991.
Dalmasso, A.P. et al. (1991) "Inhibition of complement-mediated endothelial cell cytotoxicity by decay-accelerating factor" Transplantation 52(3):530-533. Sep. 1991.
Moran, P. et al. (1992) "Human recombinant soluble decay accelerating factor inhibits complement activation in vitro and in vivo" J. Immunol. 149(5):1736-1743. Sep. 1992.
Lehto, T. et al. (1993) "Interactions of soluble CD59 with the terminal complement complexes" J. Immunol. 151(9):4941-4949. Nov. 1993.
Fodor William L.
Rollins Scott
Squinto Stephen P.
Alexion Pharmaceuticals, Inc.
Fidel Seth A.
Klee Maurice M.
Lau Kawai
Wax Robert A.
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