Nucleic acid encoding calf intestinal alkaline phosphatase

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435325, 435419, 4353201, 435196, 536 232, 536 234, C12N 1555, C12N 916

Patent

active

057078530

ABSTRACT:
The invention relates to isolated nucleic acids encoding recombinant calf intestinal alkaline phosphatase. Expression vectors and host cells transformed or transfected with such vectors are also provided. The invention further provides multifunctional polypeptides containing amino acid sequences encoding for calf intestinal alkaline phosphatase and a second amino acid sequence encoding a reagent having specific reactivity with a ligand. The recombinant calf intestinal alkaline phosphatase or its active fragments and the multifunctional polypeptides can be used in the methods for determining the presence or concentration of a ligand.

REFERENCES:
patent: 4707438 (1987-11-01), Keydar
patent: 5047507 (1991-09-01), Buchegger et al.
patent: 5055415 (1991-10-01), Imai et al.
patent: 5071761 (1991-12-01), Meyer et al.
patent: 5079141 (1992-01-01), Niskanen et al.
patent: 5079170 (1992-01-01), Rosman et al.
patent: 5079171 (1992-01-01), Senyei et al.
patent: 5084379 (1992-01-01), Calenoff et al.
patent: 5089424 (1992-02-01), Khalil et al.
patent: 5204244 (1993-04-01), Fell et al.
Berger et al., "Cloning and Sequencing of Human Intestinal Alkaline Phosphatase cDNA." Proc. Natl. Acad. Sci. USA 84:695-698 (1987).
Besman, M., and Coleman, J.E., "Isozymes of Bovine Intestinal Alkaline Phosphatase," J. Biol. Chem. 260:11190-11193 (1985).
Culp et al., "The Active-Site and Amino-Terminal Amino Acid Sequence of Bovine Intestinal Alkaline Phosphatase." Biochem. Biophys. Acta. 830:330-334 (1985).
Eliakim et al., "Differential Regulation of mRNAs Encoding for Rat Intestinal Alkaline Phosphatase." Am. J. Physiol. 259:G93-98 (1990).
Hahnel et al., "Two Alkaline Phosphatase Genes are Expressed During Early Development in the Mouse Embryo." Development. 110:555-564 (1990).
Henthorn et al., "Sequence and Characterization of the Human Intestinal Alkaline Phosphatase Gene." J. Biol. Chem. 263:12011-12019 (1988).
Hoylaerts, M.F. and Millan, J.L., "Site-directed Mutagenesis and Epitope-mapped Monoclonal Antibodies Define a Catalytically Important Conformational Difference Between Human Placental and Germ Cell Alkaline Phosphatase." Eur. J. Biochem. 202:605-616 (1991).
Knoll et al., "Nucleotide Sequence of the Human Placental Alkaline Phosphatase Gene," J. Biol. Chem. 263:12020-12027 (1988).
Manes et al., "Genomic Structure and Comparison of Mouse Tissue-Specific Alkaline Phosphatase Genes." Genomics. 8:541-554 (1990).
Millan, J.L., and Manes T., "Seminoma-derived Nagao Isozyme is Encoded by a Germ-Cell Alkaline Phosphatase Gene." Proc. Natl. Acad. Sci. USA. 85:3024-3028 (1988).
Millan, J.L., "Promoter Structure of the Human Intestinal Alkaline Phosphatase Gene." Nucl. Acids. Res. 15:10599 (1987).
Millan, J.L., "Oncodevelopmental Expression and Structure of Alkaline Phosphatase Genes." Anticancer Res. 8:995-1004 (1988).
Milstein, C., "The Amino Acid Sequence Around the Reactive Serine Residue in Alkaline Phosphatase from Escherichia coli." Biochem. J. 92:410-422 (1964).
Tsonis, et al., "A Putative Functional Domain of Human Placental Alkaline Phosphatase Predicted from Sequence Comparisons." Biochem. J. 254:623-624 (1988).
Weissig et al., "Cloning and Expression of the Bovine Intestinal Alkaline Phosphatase Gene Biochemical Characterization of the Recominant Enzyme," Biochem. J. 290(2):503-508 (1993).
Culp et al., "Expression of Bovine Intestinal Alkaline Phosphatase in Escherichia-coli, " 69th Annual Meeting of the Federation of American Societies for Experimental Biology Anaheim, CA (1985).
Sambrook et al., Molecular Cloning: A Laboratory Manual, 1989, pp. 11.3-11.19.
Millan, J. Prog. Clin. Biol. Res. 344:453-475, 1990.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Nucleic acid encoding calf intestinal alkaline phosphatase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid encoding calf intestinal alkaline phosphatase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid encoding calf intestinal alkaline phosphatase will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-325555

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.