Nucleic acid detection by the 5'-3'exonuclease activity of polym

Details Nucleic acid detection by the 5'-3'exonuclease activity of polym Nucleic acid detection by the 5'-3'exonuclease activity of polym

1993-01-05

1996-01-30

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435810, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88, C12Q 168, C12P 1934

Patent

active

054879728

ABSTRACT:
A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.

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Setlow et al., 1972, "Deoxyribonucleic Acid Polymerase: Two Distinct Enzymes in One Polypeptide" J. Biological Chemistry 247(1):224-231.
Lehman et al., 1973, "Persistence of Deoxyribonucleic Acid Polymerase I and Its 5'-3' Exonuclease Activity in PolA Mutants of Escherichia coli K12" J. Biological Chemistry 248(22):7717-7723.
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