Nucleic acid delivery

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

Reexamination Certificate

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Details

C435S320100, C424S093100

Reexamination Certificate

active

07462351

ABSTRACT:
The present invention provides formulations and methods to enhance the delivery of nucleic acids to cells. Formulations comprising dextrin polymers in combination with sugars provide enhanced delivery of nucleic acids, particularly eucaryotic expression vectors, demonstrate enhanced delivery of nucleic acids to cells in vivo. The present invention also provides methods of treatment in combination with such formulations.

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patent: 2003/0039960 (2003-02-01), Conroy et al.
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patent: WO 01/12231 (2001-02-01), None
Conroy, Susan, et al.; “In Vitro Vector Stability and Fluid Dynamics of an Intraperitoneal Solution for Delivery of Gene Therapy;” Proceedings of the American Association for Cancer Research, Mar. 2002; vol. 41; p. 524.
Engler, Heidrun, et al.; “Enhancement of Intraperitoneal Adenovirus Mediated Transgene Expression by Icodextrin in Animal Models;” Clinical Cancer Research; Nov. 2002; vol. 6; No. 11; p. 516.
Verma, Inder M., et al., “Gene Therapy—Promises, Problems and Prospects;” Nature, Sep. 18, 1997; vol. 389, pp. 239-242.
Palu, Giorgio, et al., “In Pursuit of New Developments for Gene Therapy of Human Diseases;” Biotechnology; 1999; vol. 68, pp. 1-13.
Luo, Dan, et al., “Synthetic DNA Delivery Systems;”Nature Biology; 2000; vol. 18, pp. 33-37.
Fox, Jeffrey L.; “Gene Therapy Clinical Trials Raise Troubling Questions;” ASM News, Feb. 2000; vol. 66, No. 2, pp. 1-3.

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