Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-11-22
2005-11-22
Andres, Janet (Department: 1646)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S325000, C435S410000, C530S350000, C530S399000
Reexamination Certificate
active
06967090
ABSTRACT:
The invention provides a nucleotide sequence of bovine epidermal growth factor (bEGF) and the deduced amino acid sequence of the encoded protein. The invention further provides the nucleotide sequence of mature bEGF and the deduced mature bEGF protein. The invention extends to homologous nucleic acids, proteins, and fragments functionally equivalent to the nucleotide sequence of the bEGF gene and bEGF protein, respectively. Bovine EGF may be expressed in microorganisms such asE. coliorP. pastoris, and plant hosts, such as potato. Activity of recombinant bEGF may be confirmed using a cell proliferation/DNA synthesis assay. Bovine EGF demonstrates utility in livestock and dairy productions as a supplement in farm animal feed to promote growth; to prevent or treat intestinal infections; to stimulate precocious maturation of gut cells to secrete an appropriate spectrum of digestive enzymes; and to increase nutrient absorption.
REFERENCES:
patent: 5059586 (1991-10-01), Collier et al.
patent: 5096825 (1992-03-01), Barr et al.
patent: 5753622 (1998-05-01), Buret et al.
patent: 5785990 (1998-07-01), Langrehr
patent: 2104293 (1990-04-01), None
patent: WO 88/04180 (1988-06-01), None
patent: WO 92/16626 (1992-03-01), None
Hideo, O., Akimasa, M., Yasushi, M., Kazuhide, O., Shigaki, K., Takeshi, K. and Kazuyuki, T. (1997) Rabbit epithelial cell growth factor. Database JPOP Online, Database accession No. E50021.
Salmanian, A.H., Gushchin, A., Medvedeva, T, Noori-Daloil, M.R. and Domansky. N. (1996) Synthesis and expression of the gene for human epidermal growth factor in transgenic potato plants. Biotechnology Letters 18(9):1095-1098.
Bell, G.I., Fong. N.M., Stempien. M.M., Wormsted, M.A., Caput, D., Ku, L., Urdea, M.S., Rail, L.B. and Sanchez-Pescador, R. (1986) Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization. Nucleic Acids Res. 14: 8427-8446.
Benkel, B.F. and Fong, Y. (1996) Long range-inverse PCR (LR-IPCR): extending the useful range of inverse PCR. Genetic Analysis. Biomol. Eng. 13: 123-127.
Buret, A., Gall, D.G., Olson, M.E., and Hardin, J.A. (1997) Anti-infective properties of a muscosal cytokine: epidermal growth factor (EGF). Proc. Agric. Biotechnol Workshop, AARI, p. 19.
Buret, A., Olson, M.E., Gall, D.G. and Hardin, J.A. (1998) Effects of orally administered epidermal growth factor on enteropathogenicEscherichia coliinfection in rabbits. Infection and Immunity 66(10):4917-4923.
Carpenter, G. and Cohen, S. (1979) Epidermal growth factor. Ann. Rev. Biochem. 48: 193-216.
Clare, J. J., Romanos, M.A., Rayment, F.B., Rowedder, J.E. Smith, M.A., Payne, M.M., Sreekrishna, K. and Henwood, C.A. (1991) Production of mouse epidermal growth factor in yeast: high-level secretion usingPichia pastorisstrains containing multiple gene copies Gene 105: 205-212.
Cooke, R.M., Wilkinson, A.J., Baron, M., Pastore, A., Tappin, M.J., Campbell, I.D., Gregory, H. and Sheard, B. (1987) The solution structure of human epidermal growth factor. Nature 327: 339-341.
Donovan, S.M. and Odle, J. (1994) Growth factors in milk as mediators of infant development. Ann. Rev. Nutr. 14: 147-167.
Fisher, D. (2000) Hybrid fold recognition: combining sequence derived properties with evolutionary information. Pacific Symp. Biocomputing, Hawaii, 119-130, Jan. 2000, World Scientific.
Gray, A., Dull, T.J. and Ulinch, A. (1983) Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor. Nature 303: 722-725.
Hollenberg, M.D. and Gregory, H. (1980) Epidermal growth factor-urogastrone: biological activity and receptor binding of derivatives. Mol. Pharmacol. 17: 314-320.
Kim, J.G., Vallet, J.L. and Christenson. R.K. (2001) Characterization of uterine epidermal growth factor during pregnancy in pigs. Domestic Animal Endocrinology 20(4): 253-265.
Miller, S.A. Dykes, D.D. and Polesky. H.F. (1988) A simple salting out procedure for extracting DNA from human nucleated cells Nucleic Acids Res. 16: 1215.
Nasim. M.T., Jaenicke, S., Belduz, A., Kollmus, H., Flohe, L. and McCarthy. J.E. (2000) Eukaryotic selenocystein incorporation follows a nonprocessive mechanism that competes with translational termination. J. Biol. Chem. 275: 14846-14852.
Ochman, H., Gerber, A.S. and Hartl, D.L. (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120: 621-623.
Pascall, J.C., Jones, D.S.C., Doel, S.M., Clements, J.M., Hunter, M., Fallon, T., Edwards, M. and Brown, K.D. (1991) Cloning and characterization of a gene encoding pig epidermal growth factor. J. Mol. Endocrin. 6:63-70.
Saggi, S.J., Safirstein, R. and Price, P.M. (1992) Cloning and sequencing of the rat preproepidermal growth factor cDNA: comparison with mouse and human sequences. DNA Cell Biol. 11: 481-487.
Shin, S.Y., Watanabe, M., Kako, K., Ohtaki, T. and Munekata, E. (1994) Structure-activity relationship of human epidermal growth factor (h-EGF). Life Science 55: 131-139.
Simpson, R.J., Smith, J.A., Mortiz, R.L., O'Hare, M.J., Rudland, P.S., Morrison, J.R., Lloyd, C.J., Grogo, B., Burgess, A.W. and Nice, E.C. (1985) Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity. Eur. J. Biochem. 153: 629-637.
Stewart, F., Powel, C.A., Lennard, S.N., Allen, W.R., Amet, L. and Edwards, R.M. (1994) Identification of the horse epidermal growth factor (EGF) coding sequence and its use in monitoring EGF gene expression in the endometrium of the pregnant mare. J. Mol. Endo. 12: 341-350.
Stone, N.E., Schmutz, J.J., Shang, J., Cox, D.R. and Myers, R.M. Homo sapiens chromosome 4 clone B207D4 map 4q25, complete sequence. Genbank Accession No. AC004050. submitted Jan. 28, 1998.
Tadaki, D.K. and Niyogi, S.K. (1993) The functional importance of hydrophobicity of the tyrosine at position 13 of human epidermal growth factor in receptor binding. J. Biol. Chem. 268: 10114-10119.
Tate, W.P., Mansell, J.B., Mannering, S.A., Irvine, J.H., Major, L.L. and Wilson, D. N. (1999) UGA: a dual signal for ‘stop’ and for recoding in protein synthesis. [Review]. Biochemistry 64(12): 1342-1353.
van Rooijen, G.J.H. and Moloney, M.M. (1995, Plant seed oil-bodies as carriers for foreign proteins Bio/Technology 13: 72-77.
Zijlstra, R.T., Odle, J., Hall, W.F., Petschow, B.W., Gelberg, H.B. and Litov, R.E. (1994) Effect of orally administered epidermal growth factor on intestinal recovery of neonatal pigs infected with rotavirus. J. Ped. Gastroent. Nutr. 19: 382-390.
Benkel Bernhard F.
Bilodeau-Goeseels Sylvie
John Sushil Jacob
Selinger Leonard Brent
Andres Janet
Greenlee, Winner And Sullivan, P.C.
Her Majesty the Queen in right of Canada as represented by the
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