Nucleic acid analyzing apparatus

Image analysis – Applications – Biomedical applications

Reexamination Certificate

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Reexamination Certificate

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08073219

ABSTRACT:
The present invention provides a nucleic acid analyzing apparatus which achieves highly accurate analytical ability even in single molecule DNA analysis. The nucleic acid analyzing apparatus detects locations of fluorescent bright spots in image information about light emission, deletes defective bright spots, and thereby creates intensity trace data about proper bright spots.

REFERENCES:
patent: 6791618 (2004-09-01), Shimizu
patent: 2000-180361 (2000-06-01), None
patent: 2002-185724 (2002-06-01), None
patent: 2004-101376 (2004-04-01), None
patent: 2006-337245 (2006-12-01), None
patent: 2007-010325 (2007-01-01), None
patent: 2007-322185 (2007-12-01), None
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Anger et al., “Enhancement and Quenching of Single-Molecule Fluorescence,” The American Physical Society, 2006, vol. 96, No. 11, pp. 113002-1-113002-4.
Fu et al., “Enhanced Fluorescence and Cy5-Labeled DNA Tethered to Silver Island Films: Fluorescence Images and Time-Resolved Studies Using Single-Molecule Spectroscopy,” Analytical Chemistry, vol. 78, No. 17, Sep. 1, 2006, pp. 6238-6245.
Bharadwaj et al., “Nanoplasmonic enhancement of single-molecule fluorescence,” Nanotechnology 18 (2007), pp. 1-5.
Kappeler et al., “Field Computations of Optical Antennas,” Journal of Computational and Theoretical Nanoscience, vol. 4, No. 3, 2007, pp. 686-691.
Esteban et al., “Simulation of optical near and far fields of dielectric apertureless scanning probes,” Nanotechnology 17 (2006), pp. 475-482.
Ju et al., “Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators,” Proceedings of the National Academy of Sciences of the United States of America, Dec. 26, 2006, vol. 103, No. 52, pp. 19635-19640.
Japanese Office Action issued in Japanese Patent Application No. JP 2008-093275 dated Apr. 27, 2010.

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