Nucleic acid analogue induced transcription of double stranded D

Details Nucleic acid analogue induced transcription of double stranded D Nucleic acid analogue induced transcription of double stranded D

1996-05-24

1998-11-17

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 9121, C12Q 168, C12P 1934

Patent

active

058374590

ABSTRACT:
RNA is transcribed from a double stranded DNA template by forming a complex by hybridizing to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridization.

REFERENCES:
Daube, et al. 1992, Science, vol. 258 pp. 1320-1324.
Nielsen, et al. 1991, Science, vol. 254, pp. 1497-1500.

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