Nucleic acid amplification with continuous flow emulsion

Details Nucleic acid amplification with continuous flow emulsion Nucleic acid amplification with continuous flow emulsion

2011-04-19

2011-04-19

Staples, Mark (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C239S088000

Reexamination Certificate

active

07927797

ABSTRACT:
Embodiments of the present invention are directed to methods and devices/systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.

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