Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1996-07-19
1997-10-14
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 530350, C12P 1934, C12Q 168, C07K 1300
Patent
active
056771525
ABSTRACT:
The present invention provides methods for the amplification of nucleic acids using a reversibly inactivated thermostable enzyme. The reversibly inactivated enzyme is the result of a chemical modification of the protein which inactivates the enzyme. The activity of the inactivated enzyme is recovered by an incubation of the reaction mixture at an elevated temperature prior to, or as part of, the amplification reaction. Non-specific amplification is reduced because the reaction mixture does not support the formation of extension products prior to the activating incubation.
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Birch David Edward
Laird Walter Joseph
Zoccoli Michael Anthony
Horlick Kenneth R.
Johnston George W.
Petry Douglas A.
Roche Molecular Systems Inc.
Sias Stacey R.
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