Nucleic acid amplification reaction station for disposable...

Details Nucleic acid amplification reaction station for disposable... Nucleic acid amplification reaction station for disposable...

2006-05-22

2010-10-05

Bowers, Nathan A (Department: 1797)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S286500, C435S286600, C435S287200, C435S288300, C435S288400, C435S288500, C435S305200, C435S288700, C422S105000, C422S105000

Reexamination Certificate

active

07807449

ABSTRACT:
An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.

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