Nucleic acid amplification process

Details Nucleic acid amplification process Nucleic acid amplification process

1995-02-10

1996-09-10

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 912, C12P 1934, C12Q 168

Patent

active

055545173

ABSTRACT:
This invention relates to a process for amplifying a specific nucleic acid sequence. The process involves synthesizing single-stranded RNA, single-stranded DNA and double-stranded DNA. The single-stranded RNA is a first template for a first primer, the single-stranded DNA is a second template for a second primer, and the double stranded DNA is a third template for synthesis of a plurality of copies of the first template. A sequence of the first primer or the second primer is complementary to a sequence of the specific nucleic acid and a sequence of the first primer or the second primer is homologous to a sequence of the specific nucleic acid. The amplification process may be used to increase the quantity of the specific nucleic acid sequence to allow detection, or to increase the purity of the specific nucleic acid sequence as a substitute for conventional cloning methodology.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5409818 (1990-01-01), Davey et al.
Melton, et al. Nucleic Acids Research 12(8): 7035-7056 (1984).
Lewin "Gene Expression" (1980); Wiley & Sons, New York, NY, pp. 578-582.
Maniatis, et al. "Molecular Cloning" (1982); Cold Spring Harbor Press, Cold Spring Harbor, NY, pp. 128, 214, 215.
Sankor et al. Science 240:331-334 (1989).
Beyer et al. Methods in Enzymology 152:316-325 (1987).

Affiliated with

Also associated with

Rating

Nucleic acid amplification process does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid amplification process, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid amplification process will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1319870