Nuclease-resistant RNA aptamer inhibiting replication of...

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

Reexamination Certificate

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C514S04400A, C536S023100, C536S024300, C536S024100, C536S024500, C435S006120

Reexamination Certificate

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08008272

ABSTRACT:
Disclosed is a nuclease-resistant RNA aptamer for inhibiting the replication of HCV replicon. This aptamer is capable of binding specifically to HCV NS5B and inhibiting the proliferation of the HCV replicon, and includes at least one sequence selected from a group consisting of SEQ ID NOS. 1 to 4, in which a fluoro group is substituted for 2′-hydroxy of both U (uracil) and C (cytosine) bases, and SEQ ID NO. 17, in which the fluoro group is substituted for the 2′-hydroxy of both the U (uracil) and the C (cytosine) bases, and which is tagged with a cholesteryl group at a 5′ end and with idT at a 3′ end. The RNA aptamer is useful in the diagnosis and treatment of HCV infection.

REFERENCES:
patent: 2005/0209180 (2005-09-01), Jadhav et al.
patent: 2006/0246421 (2006-11-01), Raney et al.
Shin, K-S et al., J. Microbiol. Biotechnol., vol. 16, No. 10, pp. 1634-1639 (2006).
Shin Kyung-Sook et al., ‘Inhibition of the Replication of Hepatitis C Virus Replicon with Nuclease-Resistant RNA Aptamers’, In:Journal of Microbiology and Biotechnology, Oct. 28, 2006, vol. 16 (10), pp. 1634-1639.
Antonino Biroccio et al., ‘Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase’, In:Journal of Virology, Apr. 2002, vol. 76(8), pp. 3688-3696.
Hwang Byounghoon et al., ‘Isolation of specific and high-affinity RNA aptamers against NS3 helicase domain of hepatitis C virus’, In:RNA, Jul. 9, 2004, vol. 10(8), pp. 1277-1290.

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