Nuclease resistant double-stranded ribonucleic acid

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S024100, C536S024310, C514S04400A

Reexamination Certificate

active

07928217

ABSTRACT:
This invention relates to modified double-stranded oligoribonucleic acid (dsRNA) having improved stability in cells and biological fluids, and methods of making and identifying dsRNA having improved stability, and of using the dsRNA to inhibit the expression or function of a target gene.

REFERENCES:
patent: 7345027 (2008-03-01), Tolentino et al.
patent: 2003/0143732 (2003-07-01), Fosnaugh et al.
patent: 2005/0186591 (2005-08-01), Bumcrot et al.
patent: WO 9401550 (1994-01-01), None
patent: WO 03/070918 (2003-08-01), None
patent: WO 03/099298 (2003-12-01), None
patent: WO 2004/011647 (2004-02-01), None
patent: WO 2004/029212 (2004-04-01), None
patent: WO 2004/044138 (2004-05-01), None
patent: WO 2004080406 (2004-09-01), None
patent: WO 2005014782 (2005-02-01), None
patent: WO 2005014811 (2005-02-01), None
patent: 2005097817 (2005-10-01), None
Elbashir et al. EMBO Journal, 2001, vol. 20, pp. 6877-6888.
Parrish et al. Molecular Cell Biology, 2000, vol. 6., pp. 1077-1087.
Kierzek, Ryszard. Nucleic Acids Research, vol. 20, No. 19, 1992, pp. 5073-5077.
Basbaum et al., “Focalized proteolysis: spatial and temporal regulation of extracellular matrix degradation at the cell surface”Curr. Opin. Cell Biol.8:731-738 (1996).
Benezra et al., “The Id proteins and angiogenesis”Oncogene20:8334-8341 (2001).
Birkedal-Hansen et al. “Matrix Metalloproteinases: A Review”Crit. Rev. Oral Biol. Med.4:197-250 (1993).
Boyd et al., “Invasion and metastasis”Cancer Metastasis Rev.15:77-89 (1996).
Braasch et al., “Locked Nucleic Acid (LNA) fine-tuning the recognition of DNA and RNA”Chem. Biol.8:1-7 (2001).
Braasch et al., “RNA Interference in Mammalian Cells by Chemically-Modified RNA”Biochemistry42:7967-7975 (2003).
Brinckerhoff et al., “Matrix metalloproteinases: a tail of a frog that became a prince”Nature Reviews3:207-214 (2002).
Chao et al., “BCL-2 Family: Regulators of Cell Death”Annu. Rev. Immunol.16:395-419 (1998).
Childs et al., “The MDR Superfamily of Genes and Its Biological Implications,” inImportant Advances in Oncology, ed. DeVita et al., 21-36. Philadelphia: J.B. Lippencott Co., (1994).
Chiu et al., “siRNA function in RNAi: A chemical modification analysis”RNA9:1034-1048 (2003).
Chothia et al., “The Molecular Structure of Cell Adhesion Molecules”Annu. TRev. Biochem66:823-62 (1997).
Czauderna et al., “Structural variations and stabilizing modifications of synthetic siRNAs in mammalian cells”Nucleic Acids Research31:2705-2716 (2003).
D'Ari, “Cycle-regulated genes and cell cycle regulation”Bioassays23:563-5 (2001).
Elbashir et al., “RNA interference is mediated by 21- and 22-nucleotide RNAs”EMBO J.20:6877-6888 (2001).
Fotedar et al., “Apoptosis and the cell cycle”Prog. Cell Cycle Res.2:147-63 (1996).
Gould et al., “Angiogeneses: An Expanding Universe”Human Pathol.33:1061-1063 (2002).
Hammond et al., “An RNA-Directed Nuclease Mediates Post-Transcriptional Gene Silencing inDrosophilaCells,”Nature404:293-296 (2000).
Hanahan et al., “The Hallmarks of Cancer”Cell100:57-70 (2000).
Koshkin et al., “LNA (Locked Nucleic Acids): Synthesis of the Adenine; Cytosine, Guanine, 5-Methylcytosine, Thymine and Uracil Bicyclonucleoside Monomers, Oligomerisation, and Unprecedented Nucleic Acid Recognition”Tetrahedron54:3607-3630 (1998).
Krepela, “Cysteine proteinases in tumor cell growth and apoptosis”Neoplasma48:332-349 (2001).
Matrisian, “Cancer Biology: Extracellular proteinases in malignancy”Curr. Biol.9:R776-R778 (1999).
Mendelsohn et al., “The EGF receptor family as targets for cancer therapy”Oncogene19:6550-6565 (2001).
Mignatti et al., “Biology and Biochemistry of Proteinases in Tumor Invasion”Physiol. Rev.73:161-195 (1993).
Müllauer et al., “Mutations in apoptosis genes: a pathogenetic factor for human diseases”Mutat. Res.488:211-231 (2001).
Normanno et al., “The Role of EGF-Related Peptides in Tumor Growth”Front. Biosci.6.D685-707, (2001).
Norton, “ID helix-loop-helix proteins in cell growth, differentiation and tumorigenesis”J. Cell Sci.113:3897-905 (2000).
Obika et al., “Stability and structural features of the duplexes containing nucleoside analogues with a fixed N-type conformation, 2'-O,4'-C-methyleneribonucleosides”Tetrahedron Lett.39:5401-5404 (1998).
Parise et al., “New aspects of integrin signaling in cancer”Semin. Cancer Biol.10:407-414 (2000).
Prusiner et al., “Prion Protein Biology”Cell93:337-348 (1998).
Reed,, “Mechanisms of Apoptosis”Am. J. Pathol.157:1415-1430 (2000).
Rubinstein et al. “Recent Advances in Cytokines, Cytokine Receptors and Signal Transduction”Cytokine Growth Factor Rev.9:175-81 (1998).
Safar et al., “Molecular studies of prion diseases”Prog. Brain Res.117:421-434 (1998).
Stetler-Stevenson et al., “Tumor Cell Interactions with the Extracellular Matrix During Invasion and Metastasis”Annu. Rev. Cell Biol.9:541-573 (1993).
Strasser et al., “Apoptosis Signaling”Annu. Rev. Biochem.69:217-245 (2000).
Yokota, “Tumor progression and metastasis”Carcinogenesis21:497-503 (2000).
Hammond et al., “Post-Transcriptional Gene Silencing by Double-Stranded RNA”Nature Reviews Genetics2:110-119 (2001).
Parrish et al., “Functional Anatomy of a dsRNA Trigger: Differential Requirement for the Two Trigger Strands in RNA Interference”Molecular Cell6:1077-1087 (2000).
GenBank Accession No. D89815, Hepatitis C virus genomic RNA, complete sequence, Mar. 7, 1998.
GenBank Accession No. NM—000384, Homo sapiens apolipoprotein B (including Ag(x) antigen) (APOB), mRNA, Apr. 22, 2005.
GenBank Accession No. U47298, Cloning vector pGL3-Promoter, complete sequence, Apr. 17, 2002.
GenBank Accession No. X75932, H.sapiens PLK mRNA for serine/threonine protein kinase, May 5, 1994.
Layzer et al., “In vivo activity of nuclease-resistant siRNAs”, vol. 10, No. 5, 2004, pp. 766-771.
Prakash et al., “Positional effects of chemical modification on siRNA activity”, Internet Citation, [online], Mar. 28, 2004, p. MEDI175.
Harborth et al., “Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing”, Antisense & Nucleic Acid Drug Deveopment, vol. 13, No. 2, 2003, pp. 83-105.
Elbashir, et al., “Duplexes of 21-Nucleotide RNAs Mediate RNA Interference in Cultured Mammalian Cells”, Nature, vol. 411, 2001, pp. 494-498.

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