Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-06-14
1998-06-23
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435196, 435199, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 78, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
057703701
ABSTRACT:
The invention provides nuclease protection assay comprising: (A) attaching a nucleic acid probe comprising a first nucleotide sequence to a solid surface area; (B) contacting the nucleic acid probe with a nucleic acid template under conditions that promote hybridization between complementary polynucleotides, forming a probe-template complex if the template includes a segment that is complementary to the probe; (C) contacting the probe-template complex with a nuclease effective to selectively cleave the nucleotide bonds of (1) the first nucleotide sequence when the first nucleotide sequence is single stranded or (2) mismatched regions of the first nucleotide sequence when the first nucleotide sequence is in duplex nucleic acid; and (D) detecting the presence of duplex nucleic acids formed by the probe and template nucleic acids by detecting the presence of the first nucleotide sequence.
REFERENCES:
patent: 4946773 (1990-08-01), Maniatis et al.
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5359115 (1994-10-01), Cambell et al.
patent: 5420328 (1995-05-01), Campbell
patent: 5424186 (1995-06-01), Fodor et al.
patent: 5445934 (1995-08-01), Fodor et al.
Matthews et al., Analytical Biochemistry 169:1-25, Feb. 1988.
Landgraf et al., Analytical Biochemistry 198:86-91, 1991.
Goodchild, Bioconjugate Chemistry 1(3):165-187, 1990.
Ausubel et al., Short Protocols In Molecular Biology, John Wiley & Sons, Unit 6.4, pp. 6-7 to 6-10, and Units 4.6-4.7, pp. 4-14 to 4-20, 1992.
Walmsely and Patient, Mol. Biotechnol., vol. 1, pp. 265-275 (1994).
Lau et al., Anal. Biochem., vol. 209, pp. 360-366 (1993).
Haines and Gillispie, Biotechniques, vol. 12, pp. 736-741 (1992).
Strauss and Jacobowitz, Brain Res. Mol. Brain Res., vol. 20, pp. 229-239 (1993).
Burke William J.
David Sarnoff Research Center Inc.
Tran Paul B.
Zitomer Stephanie W.
LandOfFree
Nuclease protection assays does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nuclease protection assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nuclease protection assays will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1393385