Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-12-06
2008-11-04
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C530S350000, C530S387100
Reexamination Certificate
active
07445901
ABSTRACT:
Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.
REFERENCES:
patent: 4564593 (1986-01-01), Tsukamoto et al.
patent: 5151504 (1992-09-01), Croze
patent: 5278062 (1994-01-01), Samal et al.
patent: 5346994 (1994-09-01), Chomczynski
patent: 5470971 (1995-11-01), Kondo et al.
patent: 5968784 (1999-10-01), Spinella et al.
patent: 5972613 (1999-10-01), Somack et al.
patent: 5973137 (1999-10-01), Heath
patent: 6110968 (2000-08-01), Bucala et al.
patent: 6664379 (2003-12-01), Kudlicki et al.
patent: 6869604 (2005-03-01), Rybak et al.
patent: 7163793 (2007-01-01), Kudlicki et al.
patent: 2005/0014169 (2005-01-01), Latham et al.
patent: 2007/0032418 (2007-02-01), Shapiro et al.
patent: 0896524 (2003-09-01), None
patent: WO 97/40817 (1997-11-01), None
patent: WO 2004/005663 (2004-01-01), None
patent: WO 2004/076640 (2004-09-01), None
Gene Characterization Kits ; The Stratagene Catalog p. 39 (1988).
U.S. Appl. No. 60/547,721, filed Feb. 25, 2004, Latham et al.
“Material Safety Data Sheet: Ribonucleoside Vanadyl Complex,” New England BioLabs Inc.
Allewell and Sama, “The effect of ammonium sulfate on the activity of ribonuclease a,”Biochem. Biophys. ACTA, 341:484-488, 1974.
Blackburn and Jaikhani, “Ribonuclease inhibitor from human placenta: interaction with derivatives of ribonuclease A,”J. Biol. Chem., 252:12488-12493, 1977.
Blumberg, “Creating a ribonuclease-free environment,”Methods Enzymol., 152:20-24, 1987.
Cazenave, “Idiotypic-anti-idiotypic regulation of antibody sythesis inrabbits,”Proc. Natl. Acad. Sci USA, 74:5122-5125, 1977.
Chirgwin et al., “Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease,”Biochemistry, 18:5294-5299, 1979.
Chomczynski and Sacchi, “Single step method of rna isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,”Anal. Biochem., 162:156-159, 1987.
Chomczynski, “Solubolization in formamide protects rna from degradation,”Nucleic Acids Res.,20:3791-3792, 1992.
Coburn and Mackie, “Overexpression, purification, and properties ofEscherichia coliribonuclease II,”J. Biol. Chem.,271:1048-1053, 1996.
Davis et al., Basic Methods in Molecular Biology, Elsevier Science Publishing Co. New York, NY, Section 5-2, pp. 44-50, 1986.
Deveaux et al., “Inhibition of the catalytic properties ofStaphylococcus aureusnuclease by monoclonal antibodies,”Mol. Cell Biol.,74:117-128, 1987.
Feldman et al., “Interaction of ribonuclease A with estrogen receptor from rat mammary tumor MTW9*”,The Journal of Biological Chemistry, 258:5001-5004, 1983.
Fett, et al., “A monoclonal antibody to human angiogenin. Inhibition of ribonucleolytic and angiogenic activities and localization of the antigenic epitope”,Biochemistry, 33:5421-5427, 1994.
Fransen et al., “Isolation of HIV-1 RNA from plasma: evaluation of seven different methods for extraction (part two)”,Journal of Virological Methods,76:153-157, 1998.
Gilleland and Hockett Jr., “Stability of rna molecules stored in gitc,”Biotechniques, 25:944-948, 1998.
Iverson et al., “Inhibitors of angiogenesis selectivity reduce the malignant cell load in rodent models of human myeloid leukemias,”Leukemia,16:376-381, 2002 (abstract).
Jenkins and Shapiro, “Identification of small-molecule inhibitors of human angiogenin and characterization of their binding interactions guided by computational docking,”Biochemistry, 42:6674-6687, 2003.
Jenkins et al., “Virtual Screening to Enrich Hit Lists From High-Throughput Screening: A Case Study on Small-Molecule Inhibitors of Angiogenin,”Proteins: Structure, Function, and Genetics, 50:81-93, 2003.
Jocoli and Ronald, “Inhibition of ribonuclease activity by bentonite,”Can. J. Biochem.,51:1558-1565, 1973.
Jones, “On the efficacy of commonly used ribonuclease inhibitors,”Biochem. Biophys. Res. Commun., 69:469-474, 1976.
Kao et al., “A small-molecule inhibitor of the ribonucleolytic activity of human angiogenin that possesses antitumor activity,”Proc. Natl Acad. Sci. USA, 99:10066-10071, 2002 (abstract).
Lee et al., “Primary structure of human placenta ribonuclease inhibitor,”Biochemistry, 27:8545-8553, 1988.
Lee et al., “The use of immobilized anti-ribonuclease antibodies in the isolation of polyribosomes,”Immunochemistry, 9:210-213, 1972.
Lin, “Inactivation of pancreatic ribonuclease with hydroxylamine-oxygen-cupric ion,”Biochim. et Biophys. Acta,263:680-682, 1972.
Mendelsohn and Young, “Efficacy of sodium dodecyl sulfate, diethyl pyrocarbonate, proteinase k and heparin using a sensitive ribonuclease assay,”Biochim. et Biophys. Acta,519:461-473, 1978.
Murphy et al., “A potent, cost-effective rnase inhibitor,”Biotechniques,18:1068-1073, 1995.
O'Leary, “Reducing the Impact of Endogenous Ribonucleases on Reverse Transcription-PCR Assay Systems,”Clinical Chemistry, 45(4):449-450, 1999.
Pelham and Jackson, “An efficient mRNA dependent translation system for reticulocyte lysates,”Eur. J. Biochem.,67:247-256, 1976.
Robbi and Lazarow, “Synthesis of catalase in two cell-free protein-synthesizing systems and in rat liver,”Proc. Natl. Acad. Sci. USA, 75:4344-4348, 1978.
Russo and Shapiro, “Potent inhibition of mammalian ribonucleases by 3′ 5′-pyrophosphate-linked nucleotides,”J. Biol. Chem.,274:14,902-14,908, 1999.
Russo et al., “Small Molecule Inhibitors of RNase A and Related Enzymes,”Methods Enzymology, 341:629-648, 2001.
Sambrook et al., “Molecular cloning, a laboratory manual,” pp. 7.16-7.52, 1989.
Shapiro, “Cytoplasmic Ribonuclease Inhibitor,”Methods Enzymology, 341:611-628, 2001.
Shen et al., “Poly[G] improved protein productivity of cell-free translation by inhibiting mRNase in wheat germ extract”,Journal of Biotechnology, 75:221-228, 1999.
Simpson et al., “An improved method for mRNA isolation and characterization of in vitro translation products by western blotting”,Gene, 56:161-171, 1987.
Smith et al., “Potent inhibition of ribonuclease A by oligo(vinylsulfonic acid),”J. Biol. Chem.,278:20934-20938, 2003.
Spackman et al., “The Disulfide Bonds of Ribonuclease,”J. Biol. Chem. 235:648-659, 1960.
Spickler and Mackie, “Action of rnase ii and polynucleotide phosphorylase against rnas containing stem-loops of defined structure,”J. Bacteriology,182(9): 2422-2427, 2000.
Stratagen, “Gene characterization kits,” Catalog, pp. 39, 1988.
Talib and Hearst, “Initiation of RNA synthesis in vitro by vesicular stomatitis virus: single internal initiation in the presence of aurintricarboxylic acid and vanadyl ribonucleoside complexes,”Nucleic Acids Res., 11:7031-7
Kudlicki W. Antoni
Pasloske Brittan L.
Winkler Matthew M.
Applera Corporation
Norberg Gloria L.
Whisenant Ethan
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