Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-01-16
2007-01-16
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C530S350000, C530S387100, C530S387900
Reexamination Certificate
active
10675860
ABSTRACT:
Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.
REFERENCES:
patent: 5972613 (1999-10-01), Somack et al.
patent: 5973137 (1999-10-01), Heath
patent: 6110968 (2000-08-01), Bucala et al.
patent: 6869604 (2005-03-01), Rybak et al.
Devaux et al., Molecular and Cellular Biochemistry 74 : 117-128 (1987).
Worthington Nuclease, Micrococcal (S7) from http://www.worthington-biochem.com/NFCP/default.html 8 pages.
Allewell and Sama, “The effect of ammonium sulfate on the activity of ribonuclease a,”Biochem. Biophys.ACTA, 341:484-488, 1974.
Blackburn et al., “Ribonuclease inhibitor from human placenta: interaction with derivatives of ribonuclease A,” J.Biol. Chem.252:12488-12493, 1977.
Blumberg, “Creating a ribonuclease-free environment,”Methods Enzymol.152:20-24, 1987.
Cazenave, “Idiotypic-anti-idiotypic regulation of antibody sythesis inrabbits,”Proc. Natl. Acad. Sci. USA, 74:5122-5125, 1977.
Chirgwin, et al., “Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease,”Biochemistry18:5294-5299, 1979.
Chomczynski and Sacchi, “Single step method of rna isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,”Anal. Biochem.162:156-159, 1987.
Chomczynski, “Solubolization in formamide protects rna from degradation,”Nucleic Acids Res.20:3791-3792, 1992.
Coburn and Mackie, “Overxpression, purification, and properties of escherichia coli ribonuclease II,”J. Biol. Chem.271:1048-1053, 1996.
Gilleland and Hockett Jr., “Stability of rna molecules stored in gitc,”Biotechniques25:944-948, 1998.
Jocoli and Ronald, “Inhibition of ribonuclease activity by bentonite,”Can. J. Biochem.51:1558-1565, 1973.
Jones, “On the efficacy of commonly used ribonuclease inhibitors,”Biochem. Biophys. Res. Commun.69:469-474, 1976.
Lee et al., “The use of immobilized anti-ribonuclease antibodies in the isolation of polyribosomes,”Immunochemistry,9:210-213, 1972.
Lin, “Inactivation of pancreatic ribonuclease with hydroxylamine-oxygen-cupric ion,”Biochim. et Biophys. Acta263:680-682, 1972.
Mendelsohn and Young, “Efficacy of sodium dodecyl sulfate, diethyl pyrocarbonate, proteinase k and heparin using a sensitive ribonuclease assay,”Biochim. et Biophys. Acta519:461-473, 1978.
Murphy et al., “A potent, cost-effective rnase inhibitor,”Biotechniques18:1068-1073, 1995.
O'Leary, “Reducing the Impact of Endogenous Ribonucleases on Reverse Transcription-PCR Assay Systems,”Clinical Chemistry,45(4):449-450, 1999.
Pelham and Jackson, “An efficient mRNA dependent translation system for reticulocyte lysates,”Eur. J. Biochem.67:247-256, 1976.
Russo and Shapiro, “Potent inhibition of mammalian ribonucleases by 3′ 5′-pyrophosphate-linked nucleotides,”J. Biol. Chem.274:14,902-14,908, 1999.
Sambrook, et al., “Molecular cloning, a laboratory manual,” pp. 7.16-7.52, 1989.
Spackman et al., “The Disulfide Bonds of Ribonuclease,”J. Biol. Chem.235:648-659, 1960.
Spicler and Mackie, “Action of rnase ii and polynucleotide phosphorylase against rnas containing stem-loops of defined structure,”J. Bacteriology182(9): 2422-2427, 2000.
Trent et al., “A comparison of new world alphaviruses in the western equine encephalomyelitis complex by immunochemical and oligonucleotide fingerprint techniques,”J. Gen. Virol.,47:261-282, 1980.
Wolf et al., “A mechanism of the irreversible inactivation of bovine pancreatic ribonuclease by diethylpyrocarbonate ,”Eur. J. Biochem.13:519-525, 1970.
Wu, et al., “Methods in Gene Biotechnology,” CRC Press, Boca Raton, FL, pp. 29-56, 1997.
Zale and Klibanov, “Why does ribonuclease irreversibly inactivate at high temperatures?,”Biochemistry25:5432-5444, 1986.
Co-Pending U.S. Appl. No. 10/786,875, filed Feb. 25, 2004.
Pending PCT Application No. PCT/US2004/005663 filed Feb. 25, 2004.
Pending U.S. Appl. No. 60/547,721, filed Feb. 25, 2004.
Feldman et al., “Interaction of ribonuclease A with estrogen receptor from rat mammary tumor MTW9*”,The Journal of Biological Chemistry, 258:5001-5004, 1983.
Fett, et al., “A monoclonal antibody to human angiogenin. Inhibition of ribonucleolytic and angiogenic activities and localization of the antigenic epitope”,Biochemistry, 33:5421-5427, 1994.
Fransen et al., “Isolation of HIV-1 RNA from plasma: evaluation of seven different methods for extraction (part two)”,Journal of Virological Methods, 76:153-157, 1998.
Shen et al., “Poly[G] improved protein productivity of cell-free translation by inhibiting mRNase in wheat germ extract”,Journal of Biotechnology, 75:221-228, 1999.
Simpson et al., “An improved method for mRNA isolation and characterization of in vitro translation products by western blotting”,Gene, 56:161-171, 1987.
Kudlicki W. Antoni
Pasloske Brittan L.
Winkler Matthew M.
Ambion Inc.
Fulbright & Jaworski L.L.P.
Whisenant Ethan
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