Nuclear isolation medium and procedure for separating cell nucle

Details Nuclear isolation medium and procedure for separating cell nucle Nuclear isolation medium and procedure for separating cell nucle

1984-01-30

1987-05-26

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 29, 435810, 435259, 436 8, 436 17, 436 18, 436 63, 436172, 436175, 436177, C12Q 168, C12Q 102

Patent

active

046686185

ABSTRACT:
Nuclear isolation media and procedures are described for dissociating discrete, non-agglomerated cell nuclei from animal tissue without the need to use enzyme treatments, centrifugation or the like in order to achieve the desired separation. The media facilitates separation and maintains the nuclear membrane intact and in its normal physiological environment. When a DNA-fluorochrome stain is included in the medium an essentially one step combination nuclear isolation and DNA staining procedure is used to measure DNA in tissue cells by flow cytometry. Rapid and consistent results are obtained and multiple sampling of the same tissue or comparison between whole tissues and their single cell isolates are also described.

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