Nuclear hormone receptor-interacting polypeptides and related mo

Details Nuclear hormone receptor-interacting polypeptides and related mo Nuclear hormone receptor-interacting polypeptides and related mo

1994-04-04

1998-12-08

Carlson, Karen Cochrane

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435 697, 4353201, 435325, 536 231, C12Q 168, C12P 2106, C12N 1500, C07H 1700

Patent

active

058467114

ABSTRACT:
Disclosed is a method for determining whether a test protein is capable of interacting with a nuclear hormone receptor protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including a nuclear hormone receptor protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the test protein covalently bonded to a weak gene activating moiety; and (b) determining whether the test protein increases expression of the reporter gene as an indication of its ability to interact with the nuclear hormone receptor protein. Such an interaction may be hormone dependent, hormone independent, or hormone sensitive. Also disclosed is purified DNA encoding thyroid hormone receptor-interacting proteins and the polypeptides expressed from such DNA.

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