Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-09-05
2006-09-05
Pak, Michael (Department: 1646)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C434S007000, C434S007000
Reexamination Certificate
active
07101681
ABSTRACT:
Methods for identifying modulators of nuclear hormone receptor function comprise the steps of (a) forming a mixture comprising a nuclear hormone receptor, a peptide sensor and a candidate agent, but not a natural coactivator protein of the receptor, wherein the sensor provides direct, in vitro binding to the receptor under assay conditions; (b) measuring an agent-biased binding of the sensor to the receptor; and (c) comparing the agent-biased binding with a corresponding unbiased binding of the sensor to the receptor. In particular embodiments, the sensor comprises an amphipathic alpha helix nuclear hormone interacting domain comprising a recited nuclear hormone transcriptional coactivator motif sequence, the sensor is present at sub-micromolar concentration, the binding reaction occurs in solution, the sensor comprises a fluorescent label and the measuring step comprises detecting fluorescence polarization of the label. Reagents include labeled sensor peptides and reaction mixtures consisting essentially of nuclear hormone receptor, a peptide and a candidate agent.
REFERENCES:
patent: 6020141 (2000-02-01), Pantoliano et al.
patent: WO 9710337 (1997-03-01), None
Heery et al. (1997) Nature 387:733-736.
Torchia et al. (1997) Nature 387:677-684.
Montiminy (1997) Nature 387:654-655.
Mathis (1995) Clinical Chemistry 41:1391-1397, 1997.
Harlow et al. (1988) Antibodies, Cold Spring Harbor Laboratory New York, pp. 553-616, 1988.
Baeuerle Patrick
Beckmann Holger
Chen Jin-Long
Lustig Kevin
Shan Bei
Amgen Inc.
Osman Richard Aron
Pak Michael
LandOfFree
Nuclear hormone receptor drug screens does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nuclear hormone receptor drug screens, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nuclear hormone receptor drug screens will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3605998