Nuclear factors and binding assay

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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C07K 1447

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active

056124554

ABSTRACT:
The invention provides methods and compositions for identifying pharmacological agents useful in the diagnosis or treatment of disease associated with the expression of a gene modulated by a transcription complex containing at least a human nuclear factor of activated T-cells (hNFAT). The materials include a family of hNFAT proteins, active fragments thereof, and nucleic acids encoding them. The methods are particularly suited to high-throughput screening where one or more steps are performed by a computer controlled electromechanical robot comprising an axial rotatable arm.

REFERENCES:
Li et al, PNAS, Sep. 1991, vol. 88: pp. 7739-7743.
Nolan (1994) Cell 77, 1-20.
Northrop et al. (1994) Nature 369, 497-502.
McCaffrey et al. (1993) Science 262, 7509-754.

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