Nuclear binding assay for steroid receptor functionality in canc

Details Nuclear binding assay for steroid receptor functionality in canc Nuclear binding assay for steroid receptor functionality in canc

1987-02-11

1989-02-21

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

424 3, 424 9, 435 7, 435 29, 435 34, 436501, 436503, 436504, 436813, C12Q 168, C12Q 120, G01N 33566, G01N 33567

Patent

active

048064645

ABSTRACT:
A method for rapidly determining the presence of functional cellular steroid receptors by assaying a tissue sample for nuclear steroid or antisteroid binding is disclosed which comprises treating the tissue with collagenase, incubating the isolated cells with a labelled steroid or a labelled antisteroid capable of complexing said receptors and measuring the bound nuclear radioactivity and the DNA of the isolated cellular nuclei.

REFERENCES:
patent: 4215102 (1980-07-01), Lee
patent: 4232001 (1980-11-01), Jensen et al.
patent: 4423151 (1983-12-01), Baranczuk
patent: 4433056 (1984-02-01), Baranczuk
T. C. Spelsberg et al., in "Clinical Laboratory Molecular Analyses", Grune & Stratton, Inc. (1985), at pp. 189-203.
I. Jung-Testas et al., J. Steroid Biochem., 20, 301 (1984).
R. R. Weichselbaum et al., J. Cell. Physiol., 103, 429 (1980).
D. Huchon, Ann. Endocrinol., 8, 223 (1977).
L. Laing et al., The Lancet, p. 168 (7/23/77).
A. G. Fazekas and J. K. MacFarlane, The Lancet, p. 565 (1982).
R. E. Leake et al., Brit. J. Cancer, 43, 59 (1981).
S. G. Korenman, J. Clin. Endocrinol. Metab., 28, 127 (1968).
K. J. Andersen et al., Anal. Biochem., 83, 703 (1977).
M. L. Graham, II et al., Mayo Clin. Proc., 59, 3 (1984).
P. A. Boyd et al., Biochem., 18, 3685 (1979).
P. A. Boyd-Leinen et al., Endocrinology, 111, 30 (1982).
P. A. Boyd-Leinen et al., J. Biological Chem., 259, 2411 (1984).
A. Geier et al., J. Endocrinol., 80, 281 (1979).
T. Thorsen, J. Steroid Biochem., 10, 661 (1979).
A. G. Fazekas et al., J. Steroid Biochem., 13, 613 (1980).
J. K. MacFarlane et al., Cancer, 45, 2998 (1980).
T. Thorsen and K. F. Stoa, J. Steroid Biochem., 10, 595 (1979).
Holdaway et al., Cancer, 52, 479 (1983).
Okret et al., Cancer Res., 38, 3904 (1978).
Duvivier et al., Clinica Chimica Acta, 112, 21 (1981).
K. Burton, Biochem. J., 62, 315 (1956).

Affiliated with

Also associated with

Rating

Nuclear binding assay for steroid receptor functionality in canc does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nuclear binding assay for steroid receptor functionality in canc, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nuclear binding assay for steroid receptor functionality in canc will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1521764