Nuclear binding assay for steroid receptor functionality in canc

Details Nuclear binding assay for steroid receptor functionality in canc Nuclear binding assay for steroid receptor functionality in canc

1984-09-19

1987-12-08

Nucker, Christine M.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

Utilizing isolate of tissue or organ as binding agent

436 63, 436 64, 436804, 436813, 436825, 435 6, 435 7, G01N 33567

Patent

active

047118563

ABSTRACT:
A method for rapidly determining the presence of functional cellular steroid receptors by assaying a tissue sample for nuclear steroid binding is disclosed which comprises treating the tissue with collagenase, incubating the isolated cells with a labelled steroid capable of complexing said receptors and measuring the bound radioactivity and the DNA of the isolated cellular nuclei.

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