NSP7524V restriction-modification genes

Details NSP7524V restriction-modification genes NSP7524V restriction-modification genes

1994-08-04

1995-08-15

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

43525233, 4353201, 435193, 536 232, C12N 922, C12N 1555, C12N 1570

Patent

active

054418810

ABSTRACT:
To provide Nsp7524V restriction-modification genes and a method for producing Nsp7524V restriction enzyme and Nsp7524V modification enzyme by using a novel microorganism having, introduced thereinto, plasmids containing said genes.
Nsp7524V restriction-modification genes. A method for producing Nsp7524V restriction enzyme and/or Nsp7524V modification enzyme which comprises incubating a microorganism carrying a plasmid having, integrated thereinto, Nsp7524V restriction-modification genes, and recovering the Nsp7524V restriction enzyme and/or Nsp7524V modification enzyme thus produced from the culture.
It becomes possible to efficiently produce Nsp7524V restriction enzyme and/or Nsp7524V modification enzyme which are useful in the field of genetic engineering.

REFERENCES:
patent: 5175101 (1992-12-01), Gotz et al.
patent: 5202248 (1993-04-01), Vancott
Kupper, D., et al. (1989) Nuc. Acids Res 17(3), 1077-1088.
Card, C. O., et al. (1990) Nuc. Acids Res. 18(6), 1377-1383.
Piekarowicz, A., et al. (1991) Nuc. Acids Res. 19(8), 1831-1835.
Lunnen, K. D., et al. (1988) Gene 74,25-32.
Wilson, G. G. (1988) Gene 74,281-284.
"Nostoc PCC7524, a cyanobacterium which contains five sequence-specific deoxyriboncleases", Gene 20 (1982) pp. 103-110, John Reaston et al.

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