Novel superoxide dismutase and process for preparation thereof

Details Novel superoxide dismutase and process for preparation thereof Novel superoxide dismutase and process for preparation thereof

1986-10-03

1988-09-27

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435822, C12N 902, C12R 101

Patent

active

047741856

ABSTRACT:
A novel superoxide dismutase prepared from a superoxide dismutase-producing strain of the genus Nocardiopsis. The enzyme is a tetramer with a molecular weight of about 8.8.times.10.sup.4, composed of sub-units of about 2.2.times.10.sup.4. The superoxide dismutase contains 1.56 g-atoms of iron per mole but no copper or manganese. Potassium cyanide at a concentration of 1 mM does not inhibit activity, however exponential inactivation is caused by 5 mM hydrogen peroxide.

REFERENCES:
patent: 4563349 (1986-01-01), Miyata et al.
ATCC Catalogue of Bacteria, 16th edition, 1985, pp. 123-124.
Chemical Abstracts, vol. 98, No. 5, Jan. 1983, "Purification and Properties of a Unique Superoxide Dismutase from Nocardia Asteroides".
E. M. Gregory et al, J. Bacterial., vol. 117, No. 2, pp. 456-460.

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