Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1988-10-21
1990-06-19
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 91, 435870, C12N 922, C12P 1934, C12R 135
Patent
active
049353675
ABSTRACT:
A new restriction enzyme, Mfe I, has been discovered. Mfe I recognizes the sequence CAATTG and cuts at the recognition sequence C'AATTG and generates compatible cohesive ends with EcoRI cleaved fragments. Various utilities of the enzyme have been described.
REFERENCES:
Tung, C.-S., et al. (1986) J. Biol. Chem. 261(8), 3700-3709.
Tate (1987) Nucl. Acids Res 15(16), 6739.
Halden Nancy F.
Leonard Warren J.
Wolf Julie B.
Jain Mishrilal
Patterson Jr. Charles L.
The United States of America as represented by the Department of
Wiseman Thomas G.
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