Novel recombinant DNA cosmid shuttle vectors

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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43525233, 43525235, 435320, 435849, 435889, 935 26, 935 73, 935 75, 935 80, C12N 1500, C12N 120, C12N 700

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049218018

ABSTRACT:
Novel recombinant DNA cosmid shuttle vectors and a method of using them in the construction of genomic DNA libraries are described. The vectors demonstrate the incorporation of both the size selection and in vitro packaging mechanisms of lambda into a Streptomyces-E. coli shuttle vector by the incorporation of two or more COS sequences of bacteriophage lambda.

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Chambers A. et al., Construction and Use of a Bifunctional Streptomycete Cosmid, 1984, Biochemical Society Transactions, 607th Meeting, London, vol. 12, pp. 644-645.
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Poustka et al., 1984, Proc. Natl. Acad. Sci. U.S.A. 81:4129-4133.
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