Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...
Patent
1994-08-22
1996-07-02
Caldarola, Glenn
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
For cleaning a specific substrate or removing a specific...
435221, 510321, 510392, 510393, 510530, C11D 3386
Patent
active
055319181
DESCRIPTION:
BRIEF SUMMARY
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of PCT/DK93/00074 filed Mar. 3, 1993, the contents of which are incorporated herein by reference.
TECHNICAL FIELD
This invention is in the field of detergent proteases derived from strains of Bacillus sp. More specifically, the invention is directed towards a novel alkaline protease derived from a strain of Bacillus sp. PD138. Moreover, the invention is directed towards a process for the preparation of the protease, the use of the protease as detergent enzyme, and detergent compositions comprising the protease of the invention.
BACKGROUND ART
Detergent enzymes have been marketed for more than 20 years and are now well established as normal detergent ingredients in both powder and liquid detergents all over the world. With the trend towards lower temperature washing, detergent enzyme consumption has increased during late years. Enzymes used in washing formulations comprise proteases, lipases, amylases, cellulases, as well as other enzymes, or mixtures hereof. Commercially most important are proteases.
Detergent proteases have been developed by isolation of proteases found in nature followed by testing in detergent formulations. Most detergent proteases are obtained from members of the genus Bacillus. Currently new types of proteases enter the market, offering the possibility of giving a better cost/performance ratio at various specified conditions.
Examples of commercial protease products are ALCALASE.TM., ESPERASE.TM. and SAVINASE.TM., all supplied by Novo Nordisk A/S, Denmark. These and similar enzyme products from other commercial sources are active in detergent solutions, i.e. at pH values in the range of from 8 to 11 and in the presence of sequestering agents, surfactants and bleaching agents such as sodium borate. The ALCALASE.TM. protease is produced by strains of the species Bacillus licheniformis . The ESPERASE.TM. and SAVINASE.TM. proteases are obtained by cultivation of strains of alkalophilic Bacilli.
SUMMARY OF THE INVENTION
It is an object of the present invention to provide novel detergent proteases with improved washing performance at high pH and high ion strength.
Accordingly, in its first aspect, the invention provides a protease having an apparent molecular weight of 28 kD, pl above 9.5, pH optimum at pH 11 or above (at 25.degree. C.), temperature optimum in the range of from 45.degree. to 55.degree. C. (at pH 9.5), and immunochemical properties identical or partially identical to those of a protease derived from the strain Bacillus sp. PD138, NCIMB 40338.
In a second aspect, the invention relates to an isolated biologically pure culture of a strain of Bacillus sp. represented by the strain Bacillus sp. PD138. In a more specific aspect, the invention relates to a strain of Bacillus sp. PD138, NCIMB 40338, or a mutant or a variant thereof.
In a third aspect, the invention provides a process for the preparation of the protease, which process comprises cultivation of a protease producing strain of Bacillus sp. PD138 in a suitable nutrient medium, containing carbon and nitrogen sources and inorganic salts, followed by recovery of the desired enzyme. In a more specific aspect, Bacillus sp. PD138, NCIMB 40338, or a mutant or a variant thereof, or another host organism carrying the gene encoding a protease having immunochemical properties identical or partially identical to those of the protease derived from Bacillus sp. PD138, is cultivated.
In a fourth aspect, the use of the enzyme as detergent enzyme is claimed. In more specific aspects, the invention provides detergent compositions and detergent additives comprising the protease.
In a fifth aspect, the invention provides a washing process comprising addition of the protease.
BRIEF DESCRIPTION OF DRAWINGS
The present invention is further illustrated by reference to the accompanying drawings, in which
FIG. 1 shows the relation between temperature and the proteolytic activity of an enzyme according to the invention (the enzyme preparation obtained according
REFERENCES:
patent: 4480037 (1984-10-01), Ichisima et al.
patent: 4764470 (1988-08-01), Durham et al.
patent: 4771003 (1988-09-01), Stellwag et al.
patent: 5387518 (1995-02-01), Sawayanagi et al.
Aaslyng Dorrit A.
Dambmann Claus
Lindegaard Poul
Outtrup Helle
Caldarola Glenn
Ghyka Alexander G.
Lambiris Elias J.
Novo Nordisk A S
Zelson Steve T.
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