Novel prokaryotic expression and secretion system

Details Novel prokaryotic expression and secretion system Novel prokaryotic expression and secretion system

1987-06-30

1991-09-10

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 435 698, 435 688, 4352523, 43525233, 4353201, 536 27, 935 29, 935 48, 935 22, 935 73, C12N 1531, C12N 1570, C12N 1503, C12P 2100, C12P 2102

Patent

active

050473344

ABSTRACT:
The subject invention concerns a novel and superior recombinant DNA expression/secretion system for use in prokaryotic organisms to produce useful proteins. The system can be used to have proteins secreted by an engineered prokaryotic host where normally the proteins are not secreted by the host. Also, when using the expression system, proteins are expressed at much higher levels than when the expression system is not used.

REFERENCES:
Talmadge, K., Stahl, S. and Gilbert, W. (1980), "Eukaryotic Signal Sequence Transports Insulin Antigen in Escherichia coli", Proc. Natl. Acad. Sci. U.S.A., 77:3369-3373.
Masui, Y., Mizuno, T. and Inouye, M. (1984), "Novel High-Level Expression Cloning Vehicles: 10.sup.4 -fold Amplification of Escherichia coli Minor Protein", Bio/Technology, 2:81-85.
Ghrayeb, J., Kimura, H., Takahara, M., Hsuing, H. Masui, Y. and Inouye, M. (1984), "Secretion Cloning Vectors in Escherichia coli", EMBO J. 3:2437-2442.
Watson, M. E. E. (1984), "Compilation of Published Signal Sequences", Nucl. Acids Res., 12:5145-5164.
Chan, S. J., Weiss, J., Konrad, M., White, T., Bahl, C., Yu, S.-D., Marks, D. and Steiner, D. F. (1981), "Biosynthesis and Periplasmic Segregation of Human Proinsulin in Escherichia coli", Proc. Natl. Acad. Sci. U.S.A., 78:5401-5405.
Talmadge, K. and Gilbert, W. (1982), "Cellular Location Affects Protein Stability in Escherichia coli", Proc. Natl. Acad. Sci. U.S.A., 79:1830-1833.

Affiliated with

Also associated with

Rating

Novel prokaryotic expression and secretion system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Novel prokaryotic expression and secretion system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Novel prokaryotic expression and secretion system will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-539525