Novel polypeptides and method of producing same

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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424 855, 435811, A61K 4502

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active

048554090

ABSTRACT:
A novel polypeptide which is at least equivalent in biological activity to IFN-.gamma. and is resistant to dimerization and polymerization, a transformant which carries a DNA coding for a novel polypeptide, and a method of producing a novel polypeptide from the culture of the transformant.
The DNA coding for the novel polypeptide can be produced, for example, starting with a known plasmid which contains the IFN-.gamma. gene (cDNA), namely pHITtrp1101 or pHITtrp 1201.
Insertion of this DNA into a vector followed by introduction into a host gives the transformant. An antibody column is used for the purification of the polypeptide from the culture.
The novel polypeptide produced can be used as an antiviral agent or an antitumor agent.

REFERENCES:
patent: 4604284 (1986-08-01), Kung et al.
patent: 4714611 (1987-12-01), Yasaburgo et al.
patent: 4727138 (1988-02-01), Goeddel et al.
Tanaka et al, "Expression in Escherichia coli of Chemically Synthesized Gene for the Human Immune Interferon", Nucleic Acids Research, vol. 11, No. 6, 1983, pp. 1707-1723.
Gray, P. W. et al., Nature, 295, 503-508 (1982).
Devos, R. et al., Nucleic Acids Research, 10, 2487-2501 (1982).
H. M. Johnson et al., J. Immunology, 129, 2357-2359 (1982).
D. J. Capon et al., Conference Abstracts, 3rd Annual International Congress for Interferon Research Miami, Fla., 1982.
Nikkei Biotech., 6-4-84 (No. 65).

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