Novel plasmid shuttle vectors conferring spiramycin resistance i

Details Novel plasmid shuttle vectors conferring spiramycin resistance i Novel plasmid shuttle vectors conferring spiramycin resistance i

1986-03-20

1990-02-27

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351721, 4351723, 43525232, 43525235, 435320, 435886, 536 27, 935 6, 935 9, 935 29, 935 72, 935 73, 935 75, C12N 120, C12N 1500, C12R 1465, C07H 2104

Patent

active

049045989

ABSTRACT:
A novel gene conferring resistance to spiramycin in Streptomyces griseofuscus was cloned from a genomic library of Streptomyces ambofaciens DNA. An eighteen kb fragment of S. ambofaciens DNA surrounding the spiramycin resistance gene was isolated from this library on a plasmid cosmid designated pKC514. The novel spiramycin-resistance gene can be isolated on an .about.3.4 kb Sau3AI fragment by subcloning restriction fragments obtained from the pKC514 insert DNA. This Sau3AI fragment contains all of the information required for the expression of the spiramycin resistant phenotype in Streptomyces. Vectors and transformants containing the novel spiramycin resistance gene are provided.

REFERENCES:
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Pernodet et al.; Mol. Gen. Genet. 198: 35, (1984).
Manis et al., Abstracts of the Annual Meeting of American Society for Microbiology 1984, 84th Annual Meeting, St. Louis, Mo., p. 119.
Chambers and Hunter, 1984, Biochem. Soc. Trans., 12:644-645.
Thompson et al., 1982, Gene 20:51-62.
Thompson et al., 1982, J. Bacteriol. 151:668-677.
Fujisawa and Weisblum, 1981, J. Bacteriol., 146:621-631.
Richardson et al., (1987) Gene 61: 231-241.

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