Novel method and compositions for introducting alien DNA in vivo

Details Novel method and compositions for introducting alien DNA in vivo Novel method and compositions for introducting alien DNA in vivo

1985-11-06

1988-08-09

Martinell, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435 91, 4351721, 435253, 435320, 935 23, 935 27, 935 29, 935 55, 935 56, 935 64, 935 67, 935 72, 935 73, C12N 1500, C12N 120, C12P 2100, C12P 1934

Patent

active

047627859

ABSTRACT:
Method and compositions are provided for introducing alien DNA into a nucleic acid in vivo by providing a plasmid capable of replication in a first host, which is capable of conjugation with a second host into which the alien DNA is to be introducted. The plasmid is characterized by being capable of maintenance in said first host, being incapable of maintenance in said second host, having a region of homology with DNA present in said second host, not self-transmissable but capable of mobilization by a helper plasmid and having a marker, which may be the alien DNA, for selection in said second host. The invention finds particular application for introducing alien DNA into the Ti plasmid of Agrobacterium tumefaciens for introduction into plant cells.

REFERENCES:
Hooykaas, "Plasmid Genes Essential for the Interactions of Argobacteria and Rhizobia with Plant cells", in Molecular Genetics of the Bacteria-Plant Interaction, Puhler (ed.), Springer-Verlag, Berlin, 1983, pp. 229-239.
Thomashow et al., "Integration and Organization of Ti Plasmid Sequences in Crown Gall Tumors", Cell, 19: 729, (1980).
Petit et al.: Nature, 271, 570, (1978).
Low et al.: Ann. Rev. Genet., 12, 249, (1978).
Eisenstark: Ann. Rev. Genet., 11,369, (1977).
Matzke et al.: Chem. Abstr. 95:111256u, (1981), of J. Mol. Appl. Genet., 1, 39, (1981).
Ditta et al., Proc. Natl. Acad. Sci. USA, (1980), 77:7347-7351.
Ruvkun and Ausubel, Nature, (1981), 289:85-88.

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