Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1988-05-11
1991-11-26
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4351723, 43525233, 4353201, 536 27, 935 23, 935 29, 935 56, 935 72, 935 73, C12N 1500, C12N 1570
Patent
active
050681931
ABSTRACT:
Method and compositions are provided for introducing alien DNA into a nucleic acid in vivo by providing a plasmid capable of replication in a first host, which is capable of conjugation with a second host into which the alien DNA is to be introduced. The plasmid is characterized by being capable of maintenance in said first host, being incapable of maintenance in said second host, having a region of homology with DNA present in said second host, not self-transmissable but capable of mobilization by a helper plasmid and having a marker, which may be the alien DNA, for selection in said second host. The invention finds particular application for introducing alien DNA into the Ti plasmid of Agrobacterium tumefaciens for introduction into plant cells.
REFERENCES:
Growth of the Bacterial Cell, 1983, Ingraham et al. (ed.), Sinauer Associates, Inc., Sunderland, Mass., pp. 215-217.
Ruvkun et al.; Nature 289: 85 (1981).
Matzke et al.; J. Mol. Appl. Genet. 1: 39 (1981).
Calgene Inc.
Martinell James
Rae-Venter Barbara
Rowland Bertram I.
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