Novel hybridoma cell line producing monoclonal antibodies agains

Details Novel hybridoma cell line producing monoclonal antibodies agains Novel hybridoma cell line producing monoclonal antibodies agains

1983-03-24

1987-02-10

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 68, 4351722, 435 7, 436548, C12N 500, C07K 1504

Patent

active

046422935

ABSTRACT:
The invention relates to a novel hybridoma continuous cell line that produces a monoclonal antibody of the IgG type to the glycoprotein GP-2, a subunit of the basement membrane component laminin. The hybridoma cell line is formed from a spleen cell from a Lewis rat, which has been immunized with a basement membrane antigen, fused with a mouse myeloma cell.

REFERENCES:
Chung et al. "Properties of a Basement-Membrane Related Glycoprotein Synthesized In Culture By a Mouse Embryonal Carcinoma-Derived Cell Line" 1979 Cell vol. 16, 277-287.
Fitch, John M. et al. Monoclonal Antibody Analysis of Ocular Basement Membranes During Development Developmental Biology 95 pp. 137-158 (1983).
Timpl, R. et al. "Laminin-A Glycoprotein From Basement Membranes Journal of Biological Chemistry" vol. 254, No. 19, pp. 9933-9937 (1979).
Springer, T. et al. "Monoclonal Xenogeneic Antibodies To Murine Cell Surface Antigens: Identification of Novel Leuckocyte . . . ". Differentiation Antigens Eur. J. Immunol vol. 8 pp. 539-551 (1978).
Melchers, F. et al., Ed. Lymphocyte Hybridomas Table 2 pp. XVIII-XXIII (Springer-Verlag Pub.) 1978.
Herbert, W. J. "Appendix 4 Laboratory Animal Techniques For Immunology" in Handbook of Experimental Immunology Weir, D. M. ed. pp. A4.1-A4.4 1979.

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