Novel human TNF polypeptide mutants and DNA's encoding said muta

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 4351723, 4353201, 4352523, 43525233, 935 10, 536 27, 530350, 530351, C12P 2100, C12N 1500, C07H 1512

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049904558

ABSTRACT:
A human TNF polypeptide mutant having an amino acid sequence of modified human TNF polypeptide, a DNA having a base sequence encoding the above human TNF polypeptide mutant and a method of producing the above human TNF polypeptide mutant by culturing a host transformed with a vector having inserted therein the above DNA. The above human TNF polypeptide mutant is soluble and has antitumor activity.

REFERENCES:
patent: 3723249 (1973-03-01), Kubota et al.
patent: 4775623 (1988-10-01), Katsumata et al.
Agricultural & Biological Chemistry, 36, 1675-1684 (1972).
Journal of General and Applied Microbiology 19, pp. 339-352 (1973); 16, pp. 373-391 (1970) (Kubota).
Shirai et al., Nature, vol. 313, Feb. 28, 1985, pp. 803-806.
Yamada et al., Journal of Biotechnology, vol. 3, 1985, pp. 141-153.

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