Novel Host e. coli and use thereof

Details Novel Host e. coli and use thereof Novel Host e. coli and use thereof

1986-12-29

1989-10-17

Hazel, Blondel

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351721, 4351723, 43525233, 4352528, 424 855, C12P 2100, C12R 1185

Patent

active

048746975

ABSTRACT:
E. coli deficient in protease activities, said activities being not inhibited with diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, N-.alpha.-tosyl-L-lysyl chloromethyl ketone, N-tosyl-L-phenylalanine chloromethyl ketone, ethylenediamine tetra-acetic acid, leupeptin, antipain, .alpha..sub.2 -macroglobulin or chymostatin, and being inhibited with zinc chloride or copper chloride is disclosed.
This E. coli is useful as a host for creating a transformant capable of expressing an exogenous protein or polypeptide.
The protein or polypeptide expressed by the transformant may be extracted and purified with a quite low level of decomposition.

REFERENCES:
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