Novel endonuclease and process for production thereof

Details Novel endonuclease and process for production thereof Novel endonuclease and process for production thereof

1977-07-18

1979-07-17

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C07G 7026, C12D 1306

Patent

active

041614240

ABSTRACT:
The present invention relates to novel enzymes which decompose nucleic acid and are endowed with a substrate specificity recognizing specific biological deoxyribonucleic acid and cleaving specific bonds thereof to produce fragments of deoxyribonucleic acid of specific sizes and which are obtained by culturing microorganisms belonging to genus Bacillus and purifying the cell free extract thereof by such treatment as streptomycin treatment, ammonium sulfate precipitation, ion exchange chromatography, gel filtration or combination of these processes.

REFERENCES:
patent: 3956064 (1976-05-01), Horikoshi et al.
patent: 4064011 (1977-12-01), Mayer et al.
patent: 4080261 (1978-03-01), Shibata et al.

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