Novel E. coli hybrid plasmid vector conferring sucrose fermentin

Details Novel E. coli hybrid plasmid vector conferring sucrose fermentin Novel E. coli hybrid plasmid vector conferring sucrose fermentin

1985-02-19

1989-02-21

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435320, 4351723, 935 29, C12N 120, C12N 100, C12N 1500

Patent

active

048064807

ABSTRACT:
A new hybrid plasmid vector suitable for transforming microorganisms of the genus Escherichia. This plasmid vector confers sucrose fermenting capacity to E. coli K12 cells. The plasmid vector may also be useful for carrying additional genetic information capable of controlling the production of commercially useful chemical compounds. Fermentation cultures of the microorganisms containing this plasmid vector may be propagated in a medium containing sucrose as the main carbon source, thereby increasing the plasmid stability.

REFERENCES:
Schmidt et al., J. of Bacteriol, 151: 68-76, Jul. 1982.
Prentki et al., Gene 4: 289-299, 1981.
Maniatis et al., Molecular Cloning a Laboratory Manual, Cold Spring Harbor, p. 505 (1982).

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