Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1985-10-04
1989-07-18
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 70, 435 91, 435235, 43525233, 4351723, 4353171, 435320, 4352401, 935 11, 935 29, 935 34, 935 60, 536 27, C12P 2100, C12P 1934, C12N 1500, C07H 1512
Patent
active
048493503
ABSTRACT:
The present invention presents a DNA containing a synthetic gene for expression of human epidermal cell growth factor of the DNA sequence; ##STR1## The adoption of the DNA of the present invention improves the stability of mRNA, the translation efficiency and the influence of the high-dimensional structure which is determined by the mRNA sequence, and made it possible to produce hEGF efficiently.
REFERENCES:
Urdea et al. (1983), PNAS80:7461-5.
J. Smith et al., Nucleic Acids Research, 10, 4467-4482 (1982).
M. S. Urdea et al., Pro. Natl. Acad. Sci. U.S.A., 80, 7461-7465 (1983).
Koichi Igarashi
Ryuji Marumoto
Yoshio Taniyama
Conlin David G.
Linek Ernest V.
Seidman S.
Takeda Chemical Industries Ltd.
Wiseman Thomas G.
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