Novel DNA for expression of delta-aminolevulinic acid synthetase

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435255, 435320, 435940, 435183, 935 28, 935 56, 935 69, 935 14, 935 64, C12N 1500, C12N 118, C12R 185

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049026208

ABSTRACT:
The present invention is a novel method for maintaining and selecting recombinant DNA-containing host cells wherein the DNA encoding a selectable phenotype and the DNA encoding a useful polypeptide are the same. The aforementioned DNA is useful for expressing .delta.-aminolevulinic acid synthetase (ALAS) for the ultimate expression of .delta.-aminolevulinic acid (ALA) in yeast and related organisms. The invention further comprises plasmids pIT300, pIT301, pIT302, pIT304, pIT305, pIT306 and related Saccharomyces ALA deficient transformants. ALA is a five carbon amino acid that is useful as a light dependent herbicide.

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