Novel cloning vehicles for polypeptide expression in microbial h

Details Novel cloning vehicles for polypeptide expression in microbial h Novel cloning vehicles for polypeptide expression in microbial h

1982-03-03

1987-05-19

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 435253, 435317, 935 29, 935 41, 935 43, C12P 2100, C12N 1500, C12N 120, C12N 100

Patent

active

046668365

ABSTRACT:
Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gram-negative bacterium, and also linked with an additional promoter sequence in certain cases. The methods utilize such plasmids to introduce genetic capability into microorganisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.

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